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Eriksson-Grennberg KG, Boman HG, Jansson JA, et al. Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants. J Bacteriol. 1965;90(1):54-62doi: 10.1128/jb.90.1.54-62.1965.
Eriksson-Grennberg, K. G., Boman, H. G., Jansson, J. A., & Thorén, S. (1965). Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants. Journal of bacteriology, 90(1), 54-62. https://doi.org/10.1128/jb.90.1.54-62.1965
Eriksson-Grennberg, K G, et al. "Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants." Journal of bacteriology vol. 90,1 (1965): 54-62. doi: https://doi.org/10.1128/jb.90.1.54-62.1965
Eriksson-Grennberg KG, Boman HG, Jansson JA, Thorén S. Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants. J Bacteriol. 1965 Jul;90(1):54-62. doi: 10.1128/jb.90.1.54-62.1965. PMID: 16562043; PMCID: PMC315594.
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J Bacteriol. 1965 Jul;90(1):54-62. doi: 10.1128/jb.90.1.54-62.1965.

Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants.

Journal of bacteriology

K G Eriksson-Grennberg, H G Boman, J A Jansson, S Thorén

Affiliations

  1. The Molecular Biology Group, Institute of Biochemistry, University of Uppsala, Uppsala, Sweden.

PMID: 16562043 PMCID: PMC315594 DOI: 10.1128/jb.90.1.54-62.1965

Abstract

Eriksson-Grennberg, Kerstin G. (University of Uppsala, Uppsala, Sweden), Hans G. Boman, J. A. Torbjörn Jansson, and Sone Thorén. Resistance of Escherichia coli to penicillins. I. Genetic study of some ampicillin-resistant mutants. J. Bacteriol. 90:54-62. 1965.-A number of ampicillin-resistant mutants have been isolated and characterized. Of these strains, two groups have been genetically investigated: members of one group, which are moderately resistant to ampicillin (Amp(r) (10)) carry mutations in a locus which we have designated ampA; another strain, which is resistant to high levels (50 mug/ml) of ampicillin, is a multistep mutant for which a genotype cannot yet be written. The phenotype of this strain has been designated Amp(r) (50). The location of ampA was studied by the interrupted-conjugation technique, with argF, metB, mtl, and serA as reference markers. The phenotypic expression was the same for ampA and argF. These experiments, as well as recombination without selection for ampicillin resistance, indicate that ampA is located between argF and pyrB. "Broken slopes" on the recombinant curves and failure to demonstrate cotransduction make a more accurate mapping difficult. Phage P1bt transduced ampA from resistant donors to sensitive recipient strains with a frequency of 5 x 10(-7). The ampA locus segregated in conjugation and transduction experiments with an Amp(r) (50) donor strain, but neither method gave a genetic transfer of high resistance to ampicillin. Penicillinase activity was demonstrated in two independent mutants carrying the ampA locus.

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