Display options
Cite
Baiocchi L, Tisone G, Falasca L, et al. Assembly of biliary lipids in native hepatic bile after orthotopic liver transplantation: A biochemical and ultra-structural study in humans. Hepatol Res. 2006;35(3):215-21doi: 10.1016/j.hepres.2006.03.001.
Baiocchi, L., Tisone, G., Falasca, L., Telesca, C., Di Paolo, D., Orlando, G., Furfaro, S., Anselmo, A., Carbone, M., & Angelico, M. (2006). Assembly of biliary lipids in native hepatic bile after orthotopic liver transplantation: A biochemical and ultra-structural study in humans. Hepatology research : the official journal of the Japan Society of Hepatology, 35(3), 215-21. https://doi.org/10.1016/j.hepres.2006.03.001
Baiocchi, Leonardo, et al. "Assembly of biliary lipids in native hepatic bile after orthotopic liver transplantation: A biochemical and ultra-structural study in humans." Hepatology research : the official journal of the Japan Society of Hepatology vol. 35,3 (2006): 215-21. doi: https://doi.org/10.1016/j.hepres.2006.03.001
Baiocchi L, Tisone G, Falasca L, Telesca C, Di Paolo D, Orlando G, Furfaro S, Anselmo A, Carbone M, Angelico M. Assembly of biliary lipids in native hepatic bile after orthotopic liver transplantation: A biochemical and ultra-structural study in humans. Hepatol Res. 2006 Jul;35(3):215-21. doi: 10.1016/j.hepres.2006.03.001. Epub 2006 Apr 19. PMID: 16621688.
Copy Download .nbib Format: NLM
Share it on

Hepatol Res. 2006 Jul;35(3):215-21. doi: 10.1016/j.hepres.2006.03.001. Epub 2006 Apr 19.

Assembly of biliary lipids in native hepatic bile after orthotopic liver transplantation: A biochemical and ultra-structural study in humans.

Hepatology research : the official journal of the Japan Society of Hepatology

Leonardo Baiocchi, Giuseppe Tisone, Laura Falasca, Claudia Telesca, Daniele Di Paolo, Giuseppe Orlando, Sonia Furfaro, Alessandro Anselmo, Marco Carbone, Mario Angelico

Affiliations

  1. Chair of Gastroenterology, University of "Tor Vergata", Rome, Italy.

PMID: 16621688 DOI: 10.1016/j.hepres.2006.03.001

Abstract

BACKGROUND: In gallbladder bile, lipids aggregate as micelles and vesicles, yet the presence of lamellae remains controversial. Little is known on lipid assembly in dilute hepatic bile. Liver transplantation represents a condition in which bile is diluted immediately after transplant and tends to normalize thereafter.

AIM: To study biliary lipidic carriers after liver transplantation in relation to the increasing bile lipid concentration.

METHODS: Three bile samples were harvested from six patients (3M/3F) with normal post-transplant outcome: sample 1 at days 2-3, sample 2 at 1 week, and sample 3 at 2 weeks after transplant. Samples were analyzed by biochemical, morphological and quasi-elastic light scattering methodology.

RESULTS: Lipid concentration increased from 0.6g/dl at day 2-3 to 3.6g/dl at week 2. Electron microscopy showed the presence of unilamellar vesicles in all samples. Large amorphous particles interpreted as proteic aggregates were also present at day 2-3, while lamellae coexisted with vesicles later. Quasi-elastic light scattering data were consistent with electron microscopic findings. Liquid crystals were observed at polarizing microscopy with increasing bile lipid concentration.

CONCLUSIONS: Normalization of biliary lipid secretion after liver transplantation is associated with: (i) increased proportion of vesicles and reduction of their size; (ii) presence of lamellae.

Publication Types