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Tanaka N, Takeuchi T, Neri QV, et al. Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. J Transl Med. 2006;4:20doi: 10.1186/1479-5876-4-20.
Tanaka, N., Takeuchi, T., Neri, Q. V., Sills, E. S., & Palermo, G. D. (2006). Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. Journal of translational medicine, 420. https://doi.org/10.1186/1479-5876-4-20
Tanaka, Noriko, et al. "Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model." Journal of translational medicine vol. 4 (2006): 20. doi: https://doi.org/10.1186/1479-5876-4-20
Tanaka N, Takeuchi T, Neri QV, Sills ES, Palermo GD. Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model. J Transl Med. 2006 May 08;4:20. doi: 10.1186/1479-5876-4-20. PMID: 16681851; PMCID: PMC1479373.
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J Transl Med. 2006 May 08;4:20. doi: 10.1186/1479-5876-4-20.

Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model.

Journal of translational medicine

Noriko Tanaka, Takumi Takeuchi, Queenie V Neri, Eric Scott Sills, Gianpiero D Palermo

Affiliations

  1. Center for Reproductive Medicine and Infertility, Weill Medical College of Cornell University, New York, NY 10021, USA. [email protected]

PMID: 16681851 PMCID: PMC1479373 DOI: 10.1186/1479-5876-4-20

Abstract

BACKGROUND: To evaluate embryonic stem cell (ESC) harvesting methods with an emphasis on derivation of ESC lines without feeder cells or sera. Using a murine model, laser-assisted blastocyst dissection was performed and compared to conventional immunosurgery to assess a novel laser application for inner cell mass (ICM) isolation.

METHODS: Intact blastocysts or isolated ICMs generated in a standard mouse strain were plated in medium with or without serum to compare ESC harvesting efficiency. ESC derivation was also undertaken in a feeder cell-free culture system.

RESULTS: Although ICM growth and dissociation was comparable irrespective of the media components, an enhanced ESC harvest was observed in our serum-free medium (p < 0.01). ESC harvest rate was not affected by ICM isolation technique but was attenuated in the feeder cell-free group.

CONCLUSION: Achieving successful techniques for human ESC research is fundamentally dependent on preliminary work using experimental animals. In this study, all experimentally developed ESC lines manifested similar features to ESCs obtained from intact blastocysts in standard culture. Cell/sera free murine ESC harvest and propagation are feasible procedures for an embryology laboratory and await refinements for translation to human medical research.

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