Display options
Cite
Weber J, Begin M, Khittoo G. Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants. J Virol. 1975;15(5):1049-56doi: 10.1128/JVI.15.5.1049-1056.1975.
Weber, J., Begin, M., & Khittoo, G. (1975). Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants. Journal of virology, 15(5), 1049-56. https://doi.org/10.1128/JVI.15.5.1049-1056.1975
Weber, J, et al. "Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants." Journal of virology vol. 15,5 (1975): 1049-56. doi: https://doi.org/10.1128/JVI.15.5.1049-1056.1975
Weber J, Begin M, Khittoo G. Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants. J Virol. 1975 May;15(5):1049-56. doi: 10.1128/JVI.15.5.1049-1056.1975. PMID: 16789144; PMCID: PMC354558.
Copy Download .nbib Format: NLM
Share it on

J Virol. 1975 May;15(5):1049-56. doi: 10.1128/JVI.15.5.1049-1056.1975.

Genetic Analysis of Adenovirus Type 2 II. Preliminary Phenotypic Characterization of Temperature-Sensitive Mutants.

Journal of virology

J Weber, M Begin, G Khittoo

Affiliations

  1. Department de Microbiologie, Centre Hospitalier Universitaire, Sherbrooke, Québec, Canada.

PMID: 16789144 PMCID: PMC354558 DOI: 10.1128/JVI.15.5.1049-1056.1975

Abstract

The properties of temperature-sensitive mutants of adenovirus type 2 representing 12 complementation groups were studied. All mutants were normal with respect to adsorption as measured by viral inclusion formation and viral DNA synthesis as shown by velocity sedimentation in alkaline sucrose gradients. One mutant, however, formed viral inclusions of altered morphology at the nonpermissive temperature. The synthesis of the major capsid proteins was examined by immunodiffusion. On this basis, the complementation groups could be arranged as follows: (i) one group was negative for all three proteins; (ii) three groups failed to synthesize penton bases; (iii) eight groups were positive for hexons, pentons, and fibers. The assembly of virus particles at 39 C was examined by equilibrium sedimentation in CsCl; three groups were found defective, whereas two of the penton-negative groups were positive for virion production. Tests of the thermolability of virions at 50 C revealed eight groups labile whereas the remainder were insensitive to heat inactivation. None of five mutants inoculated in newborn rats induced tumors, although three of them were capable of in vitro transformation.

References

  1. J Virol. 1975 Jan;15(1):1-7 - PubMed
  2. Virology. 1970 Oct;42(2):341-58 - PubMed
  3. Appl Microbiol. 1972 May;23(5):1025-6 - PubMed
  4. Virology. 1972 Aug;49(2):426-38 - PubMed
  5. Prog Exp Tumor Res. 1973;18:1-55 - PubMed
  6. J Virol. 1973 Sep;12(3):663-4 - PubMed
  7. J Gen Virol. 1972 Jul;16(1):99-102 - PubMed
  8. J Gen Virol. 1972 Dec;17(3):265-79 - PubMed
  9. J Virol. 1972 Oct;10(4):801-9 - PubMed
  10. J Virol. 1973 Mar;11(3):449-59 - PubMed
  11. J Virol. 1973 Aug;12(2):241-52 - PubMed
  12. Virology. 1974 Jul;60(1):192-9 - PubMed
  13. Virology. 1974 Aug;60(2):419-30 - PubMed
  14. J Gen Virol. 1974 Aug;24(2):247-59 - PubMed
  15. J Virol. 1969 Feb;3(2):198-204 - PubMed
  16. Virology. 1969 Aug;38(4):587-606 - PubMed
  17. J Lab Clin Med. 1958 Nov;52(5):784-7 - PubMed
  18. Nature. 1960 May 14;186:571-2 - PubMed

Publication Types