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Janasek D, Schilling M, Franzke J, et al. Isotachophoresis in free-flow using a miniaturized device. Anal Chem. 2006;78(11):3815-9doi: 10.1021/ac060063l.
Janasek, D., Schilling, M., Franzke, J., & Manz, A. (2006). Isotachophoresis in free-flow using a miniaturized device. Analytical chemistry, 78(11), 3815-9. https://doi.org/10.1021/ac060063l
Janasek, Dirk, et al. "Isotachophoresis in free-flow using a miniaturized device." Analytical chemistry vol. 78,11 (2006): 3815-9. doi: https://doi.org/10.1021/ac060063l
Janasek D, Schilling M, Franzke J, Manz A. Isotachophoresis in free-flow using a miniaturized device. Anal Chem. 2006 Jun 01;78(11):3815-9. doi: 10.1021/ac060063l. PMID: 16737242.
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Anal Chem. 2006 Jun 01;78(11):3815-9. doi: 10.1021/ac060063l.

Isotachophoresis in free-flow using a miniaturized device.

Analytical chemistry

Dirk Janasek, Michael Schilling, Joachim Franzke, Andreas Manz

Affiliations

  1. Institute for Analytical Sciences Dortmund and Berlin, Bunsen-Kirchhoff-Strasse 11, D-44139 Dortmund, Germany. [email protected]

PMID: 16737242 DOI: 10.1021/ac060063l

Abstract

For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic separation of a reaction mixture of myoglobin and fluoresceinisothiocyanate as a fluorescence label has shown the potential of this method for on-line sample preparation.

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