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App AA, Gerosa MM. A soluble fraction requirement in the transfer reaction of protein synthesis by rice embryo ribosomes. Plant Physiol. 1966;41(9):1420-4doi: 10.1104/pp.41.9.1420.
App, A. A., & Gerosa, M. M. (1966). A soluble fraction requirement in the transfer reaction of protein synthesis by rice embryo ribosomes. Plant physiology, 41(9), 1420-4. https://doi.org/10.1104/pp.41.9.1420
App, A A, and Gerosa, M M. "A soluble fraction requirement in the transfer reaction of protein synthesis by rice embryo ribosomes." Plant physiology vol. 41,9 (1966): 1420-4. doi: https://doi.org/10.1104/pp.41.9.1420
App AA, Gerosa MM. A soluble fraction requirement in the transfer reaction of protein synthesis by rice embryo ribosomes. Plant Physiol. 1966 Nov;41(9):1420-4. doi: 10.1104/pp.41.9.1420. PMID: 16656417; PMCID: PMC550547.
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Plant Physiol. 1966 Nov;41(9):1420-4. doi: 10.1104/pp.41.9.1420.

A soluble fraction requirement in the transfer reaction of protein synthesis by rice embryo ribosomes.

Plant physiology

A A App, M M Gerosa

Affiliations

  1. Boyce Thompson Institute for Plant Research, Yonkers, New York.

PMID: 16656417 PMCID: PMC550547 DOI: 10.1104/pp.41.9.1420

Abstract

The requirements for the transfer of (14)C-phenylalanine from yeast soluble ribonucleic acid to protein in vitro by rice (Oryza sativa L. var. Bluebonnet) ribosomes have been investigated. An absolute requirement for polyuridylic acid, 2-mercaptoethanol, guanosine triphosphate, magnesium, and potassium or ammonium ions and ribosomes has been demonstrated. Ribosomes washed in 0.5% sodium deoxycholate also required the presence of rice supernatant. The optimum concentration of magnesium ion for the reaction was approximately 7 mm, while 60 mm of either ammonium or potassium ion gave maximum transfer of phenylalanine in this heterologous system. The optimum concentration of guanosine triphosphate required varied with the presence or absence of the phosphoenolpyruvate-pyruvate kinase generating system. Without the system, the optimum concentration was 1.5 mm, but in its presence the optimum was approximately 0.1 mm.

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