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Plant Physiol. 1967 Sep;42(9):1179-86. doi: 10.1104/pp.42.9.1179.

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves.

Plant physiology

F Parenti, M M Margulies

Affiliations

  1. Radiation Biology Laboratory, Smithsonian Institution, Washington, D. C. 20560.

PMID: 16656637 PMCID: PMC1086699 DOI: 10.1104/pp.42.9.1179

Abstract

The incorporation of uniformly labeled leucine-(14)C into protein by a chloroplast containing fraction from developing primary leaves of bean is reported. Chloroplasts, obtained from week old plants grown in darkness, and then illuminated with white light for 12 hours, were shown to be the principal sites of incorporating activity. Incorporation may continue for 2 hours. Rates of up to 50 mumumole leucine incorporated per mg protein per hour are observed when a 1 hour assay period is used. Incorporation is only partially sensitive to ribonuclease.

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