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Meudt WJ, Gaines TP. Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products. Plant Physiol. 1967;42(10):1395-9doi: 10.1104/pp.42.10.1395.
Meudt, W. J., & Gaines, T. P. (1967). Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products. Plant physiology, 42(10), 1395-9. https://doi.org/10.1104/pp.42.10.1395
Meudt, W J, and Gaines, T P. "Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products." Plant physiology vol. 42,10 (1967): 1395-9. doi: https://doi.org/10.1104/pp.42.10.1395
Meudt WJ, Gaines TP. Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products. Plant Physiol. 1967 Oct;42(10):1395-9. doi: 10.1104/pp.42.10.1395. PMID: 16656668; PMCID: PMC1086736.
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Plant Physiol. 1967 Oct;42(10):1395-9. doi: 10.1104/pp.42.10.1395.

Studies on the oxidation of indole-3-acetic Acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic Acid oxidation products.

Plant physiology

W J Meudt, T P Gaines

Affiliations

  1. Crops Research Division, Agricultural Research Service, United States Department of Agriculture, Plant Industry Station, Beltsville, Maryland 20705.

PMID: 16656668 PMCID: PMC1086736 DOI: 10.1104/pp.42.10.1395

Abstract

The method described here is based on a brief report by Harley-Mason and Archer. It involves the use of p-dimethylaminocinnamaldehyde (DMACA), a vinylogue of Ehrlich's reagent, as a color reagent for indoles. Colorimetric analyses of indoleacetic acid (IAA) oxidation reaction mixtures were made with the DMACA reagent as a solution rather than a spray. DMACA reagent will yield a wine-red color with IAA oxidation products in solution. Under similar conditions DMACA reacts with authentic IAA to yield only slight coloration at best. In comparison with other indoles, DMACA is more relative with IAA oxidation reaction products than either Salkowski or Ehrlich's reagents. Data discussed support a concept that the color produced with DMACA is due to the presence of tautomeric oxidation product(s) of IAA.

References

  1. Arch Biochem Biophys. 1956 Sep;64(1):193-216 - PubMed
  2. Biochemistry. 1965 Jan;4:144-58 - PubMed
  3. Science. 1955 Jul 29;122(3161):187-8 - PubMed

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