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McCown BH, Hall TC, Beck GE. Plant Leaf and Stem Proteins. II. Isozymes and Environmental Cabbage. Plant Physiol. 1969;44(2):210-6doi: 10.1104/pp.44.2.210.
McCown, B. H., Hall, T. C., & Beck, G. E. (1969). Plant Leaf and Stem Proteins. II. Isozymes and Environmental Cabbage. Plant physiology, 44(2), 210-6. https://doi.org/10.1104/pp.44.2.210
McCown, B H, et al. "Plant Leaf and Stem Proteins. II. Isozymes and Environmental Cabbage." Plant physiology vol. 44,2 (1969): 210-6. doi: https://doi.org/10.1104/pp.44.2.210
McCown BH, Hall TC, Beck GE. Plant Leaf and Stem Proteins. II. Isozymes and Environmental Cabbage. Plant Physiol. 1969 Feb;44(2):210-6. doi: 10.1104/pp.44.2.210. PMID: 16657048; PMCID: PMC396064.
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Plant Physiol. 1969 Feb;44(2):210-6. doi: 10.1104/pp.44.2.210.

Plant Leaf and Stem Proteins. II. Isozymes and Environmental Cabbage.

Plant physiology

B H McCown, T C Hall, G E Beck

Affiliations

  1. Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.

PMID: 16657048 PMCID: PMC396064 DOI: 10.1104/pp.44.2.210

Abstract

The activity of 10 enzymes separated by acrylamide disc gel electrophoresis of leaf and stem extracts from Dianthus grown under summer and winter conditions was studied. While banding was constant and highly reproducible under each environment, differences between the 3 cultivars and between the tissues were evident. No significant differences in the isozyme patterns of glutamate dehydrogenase, 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase, malate dehydrogenase, and catalase were observed between the 2 environments. Loss of activity was observed under winter conditions with amylase and lactate dehydrogenase and loss of certain isozymic components was evident with acid phosphatase and esterase. Prominent changes were observed in peroxidase isozymes, the hardy cultivars developing additional isozymic components under winter conditions. Only minor changes in the total protein banding were seen. The enzymes showed considerable stability in those tissues killed by the freezing conditions.

References

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