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Biochem J. 1966 Dec;101(3):711-6. doi: 10.1042/bj1010711.

Separation of antigens by immunological specificity: Use of cellulose-linked antibodies as immunosorbents.

The Biochemical journal

R G Gallop, B T Tozer, J Stephen, H Smith

Affiliations

  1. Microbiological Research Establishment, Porton Down, Wilts.

PMID: 16742449 PMCID: PMC1270177 DOI: 10.1042/bj1010711

Abstract

1. Immunosorbents were prepared by coupling activated aminocellulose with the gamma-globulin concentrates of antisera prepared against ovalbumin and human serum albumin. 2. The immunosorbents were low in solubility, but high in capacity for homologous antigens. 3. The high specificity of these immunosorbents was demonstrated by their use in fractionating various mixtures of fluorescent ovalbumin, (131)I-labelled human serum albumin, lysozyme and ribonuclease.

References

  1. Arch Biochem Biophys. 1957 Oct;71(2):377-85 - PubMed
  2. Nature. 1959 Apr 11;183(4667):1055 - PubMed
  3. Proc Natl Acad Sci U S A. 1951 Sep;37(9):575-8 - PubMed
  4. J Immunol. 1963 Mar;90:438-43 - PubMed
  5. Nature. 1964 Aug 8;203:648-9 - PubMed
  6. Nature. 1964 Oct 17;204:265-6 - PubMed
  7. Immunology. 1964 Mar;7:111-7 - PubMed
  8. Biochim Biophys Acta. 1963 Nov 15;78:516-27 - PubMed
  9. Anal Biochem. 1963 Oct;6:328-34 - PubMed
  10. Br Med Bull. 1963 Sep;19:183-91 - PubMed
  11. Biochem J. 1962 Jul;84:74-80 - PubMed
  12. Biokhimiia. 1961 Sep-Oct;26:934-42 - PubMed
  13. Biochem J. 1961 Mar;78:457-72 - PubMed
  14. Biochem J. 1960 Oct;77:12-7 - PubMed
  15. Biochem J. 1966 Dec;101(3):717-20 - PubMed

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