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Kornberg HL, Soutar AK. Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities. Biochem J. 1973;134(2):489-98doi: 10.1042/bj1340489.
Kornberg, H. L., & Soutar, A. K. (1973). Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities. The Biochemical journal, 134(2), 489-98. https://doi.org/10.1042/bj1340489
Kornberg, H L, and Soutar, A K. "Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities." The Biochemical journal vol. 134,2 (1973): 489-98. doi: https://doi.org/10.1042/bj1340489
Kornberg HL, Soutar AK. Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities. Biochem J. 1973 Jun;134(2):489-98. doi: 10.1042/bj1340489. PMID: 16742809; PMCID: PMC1177835.
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Biochem J. 1973 Jun;134(2):489-98. doi: 10.1042/bj1340489.

Utilization of gluconate by Escherichia coli. Induction of gluconate kinase and 6-phosphogluconate dehydratase activities.

The Biochemical journal

H L Kornberg, A K Soutar

Affiliations

  1. Department of Biochemistry, School of Biological Sciences, University of Leicester, Leicester LE1 7RH, U.K.

PMID: 16742809 PMCID: PMC1177835 DOI: 10.1042/bj1340489

Abstract

1. A mutant of Escherichia coli, devoid of phosphopyruvate synthetase, glucosephosphate isomerase and 6-phosphogluconate dehydrogenase activities, grew readily on gluconate and inducibly formed an uptake system for gluconate, gluconate kinase and 6-phosphogluconate dehydratase while doing so. 2. This mutant also grew on glucose 6-phosphate and inducibly formed 6-phosphogluconate dehydratase; however, the formation of the gluconate uptake system and gluconate kinase was not induced under these conditions. 3. The use of the Entner-Doudoroff pathway for the dissimilation of 6-phosphogluconate, derived from either gluconate or glucose 6-phosphate, by this mutant was also demonstrated by the accumulation of 2-keto-3-deoxy-6-phosphogluconate (3-deoxy-6-phospho-l-glycero-2-hexulosonate) from both these substrates in a similar mutant that also lacked phospho-2-keto-3-deoxygluconate aldolase activity. 4. Glucose 6-phosphate inhibits the continued utilization of fructose by cultures of the mutants growing on fructose, as it does in wild-type E. coli. 5. The mutants do not use glucose for growth. This is shown to be due to insufficiency of phosphopyruvate, which is required for glucose uptake.

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