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Ghassemifar R, Schultz GS, Tarnuzzer RW, et al. Alpha-smooth muscle actin expression in rat and mouse mesenteric wounds after transforming growth factor-beta1 treatment. Wound Repair Regen. 1997;5(4):339-47doi: 10.1046/j.1524-475X.1997.50408.x.
Ghassemifar, R., Schultz, G. S., Tarnuzzer, R. W., Salerud, G., & Franzén, L. E. (1997). Alpha-smooth muscle actin expression in rat and mouse mesenteric wounds after transforming growth factor-beta1 treatment. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 5(4), 339-47. https://doi.org/10.1046/j.1524-475X.1997.50408.x
Ghassemifar, R, et al. "Alpha-smooth muscle actin expression in rat and mouse mesenteric wounds after transforming growth factor-beta1 treatment." Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society vol. 5,4 (1997): 339-47. doi: https://doi.org/10.1046/j.1524-475X.1997.50408.x
Ghassemifar R, Schultz GS, Tarnuzzer RW, Salerud G, Franzén LE. Alpha-smooth muscle actin expression in rat and mouse mesenteric wounds after transforming growth factor-beta1 treatment. Wound Repair Regen. 1997 Oct-Dec;5(4):339-47. doi: 10.1046/j.1524-475X.1997.50408.x. PMID: 16984444.
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Wound Repair Regen. 1997 Oct-Dec;5(4):339-47. doi: 10.1046/j.1524-475X.1997.50408.x.

Alpha-smooth muscle actin expression in rat and mouse mesenteric wounds after transforming growth factor-beta1 treatment.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

R Ghassemifar, G S Schultz, R W Tarnuzzer, G Salerud, L E Franzén

Affiliations

  1. Department of Pathology II, University Hospital, Linköping, Sweden.

PMID: 16984444 DOI: 10.1046/j.1524-475X.1997.50408.x

Abstract

Rat mesenteric perforations heal by contraction within 5 to 7 days, whereas mouse mesenteric perforations seldom close within 3 weeks unless stimulated by transforming growth factor-beta1. In this article, we quantified the expression of alpha-smooth muscle actin by quantitative-reverse transcription-polymerase chain reaction and the orientation of actin filaments at the wound margin by Fourier transformation image analysis after treatment with transforming growth factor-beta1. The expression of transforming growth factor-beta1 and its type II receptor was also assessed. Actin filaments were shown to increase with time at the wound margin in both species and the expression of alpha-smooth muscle actin mRNA increased simultaneously. Transforming growth factor-beta1 enhanced the alpha-smooth muscle actin expression four to five times in rats and three to four times in mice on day 5, but the number of copies expressed per cell was 15-fold higher in rats than in mice. Transforming growth factor-beta1 was down-regulated after wounding in free peritoneal cells of rats, but maintained until day 5 in transforming growth factor-beta1-treated mice. The main finding of this study was that untreated, normal rats expressed substantially more alpha-smooth muscle actin than mice. After treatment with transforming growth factor-beta1, this expression increased similarly in both species. It can be hypothesized that normal closure of mesenteric perforations requires a minimum level of actin expression. This level is not reached in normal mice, but is exceeded after stimulation. Perforations in the rat always close, because the alpha-smooth muscle actin expression is always above this level.

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