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Yang ST, Wilken LO, Clark CR. Liquid chromatographic determination of the pH-dependent degradation of eseroline--hydrolysis product of physostigmine. J Pharm Biomed Anal. 1987;5(4):383-93doi: 10.1016/0731-7085(87)80045-6.
Yang, S. T., Wilken, L. O., & Clark, C. R. (1987). Liquid chromatographic determination of the pH-dependent degradation of eseroline--hydrolysis product of physostigmine. Journal of pharmaceutical and biomedical analysis, 5(4), 383-93. https://doi.org/10.1016/0731-7085(87)80045-6
Yang, S T, et al. "Liquid chromatographic determination of the pH-dependent degradation of eseroline--hydrolysis product of physostigmine." Journal of pharmaceutical and biomedical analysis vol. 5,4 (1987): 383-93. doi: https://doi.org/10.1016/0731-7085(87)80045-6
Yang ST, Wilken LO, Clark CR. Liquid chromatographic determination of the pH-dependent degradation of eseroline--hydrolysis product of physostigmine. J Pharm Biomed Anal. 1987;5(4):383-93. doi: 10.1016/0731-7085(87)80045-6. PMID: 16867508.
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J Pharm Biomed Anal. 1987;5(4):383-93. doi: 10.1016/0731-7085(87)80045-6.

Liquid chromatographic determination of the pH-dependent degradation of eseroline--hydrolysis product of physostigmine.

Journal of pharmaceutical and biomedical analysis

S T Yang, L O Wilken, C R Clark

Affiliations

  1. Department of Pharmacal Sciences, School of Pharmacy, Auburn University, Auburn, AL 36849, USA.

PMID: 16867508 DOI: 10.1016/0731-7085(87)80045-6

Abstract

The stability of eseroline, the hydrolysis product of physostigmine under aerobic conditions, was studied by liquid chromatography. A reversed-phase, ion-pair technique was used to separate eseroline from its degradation products. The degradation of eseroline in phosphate buffer solutions of pH 6.91, 7.40, 7.98, 8.41 and 8.94 appears to follow first-order kinetics; the rate constant increased with an increase in pH. The degradation appears to follow specific base catalysis.

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