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Rollman B, Van der Auwera P, Tulkens PM. Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay. J Pharm Biomed Anal. 1986;4(1):53-61doi: 10.1016/0731-7085(86)80023-1.
Rollman, B., Van der Auwera, P., & Tulkens, P. M. (1986). Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay. Journal of pharmaceutical and biomedical analysis, 4(1), 53-61. https://doi.org/10.1016/0731-7085(86)80023-1
Rollman, B, et al. "Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay." Journal of pharmaceutical and biomedical analysis vol. 4,1 (1986): 53-61. doi: https://doi.org/10.1016/0731-7085(86)80023-1
Rollman B, Van der Auwera P, Tulkens PM. Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay. J Pharm Biomed Anal. 1986;4(1):53-61. doi: 10.1016/0731-7085(86)80023-1. PMID: 16867630.
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J Pharm Biomed Anal. 1986;4(1):53-61. doi: 10.1016/0731-7085(86)80023-1.

Dibekacin assay in serum by automated fluorescence polarization immunoassay (Abbott Tdx): comparison with high-performance liquid chromatography, substrate-labelled fluorescent immunoassay and radioimmunoassay.

Journal of pharmaceutical and biomedical analysis

B Rollman, P Van der Auwera, P M Tulkens

Affiliations

  1. Université Catholique de Louvain, Laboratoire de Chimie Physiologique et de Chimie Analytique, 1200 Brussels, Belgium.

PMID: 16867630 DOI: 10.1016/0731-7085(86)80023-1

Abstract

An immunoassay based on fluorescence polarization detection (FPIA) has been recently adapted for dibekacin. This has been compared with a reference method (high-performance liquid chromatography), and two other methods used in clinical laboratories for monitoring this aminoglycoside, namely substrate-labelled fluorescent immunoassay (SLFIA) and radioimmunoassay (RIA). FPIA was fast and more reliable than SLFIA or RIA, and offered therefore superior performance. However, its nominal cost per assay is high.

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