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Wideman J, Pan YC, Lahm HW, et al. Characterization of small amounts of peptides and proteins. J Pharm Biomed Anal. 1984;2(2):281-7doi: 10.1016/0731-7085(84)80078-3.
Wideman, J., Pan, Y. C., Lahm, H. W., Chang, M., & Stein, S. (1984). Characterization of small amounts of peptides and proteins. Journal of pharmaceutical and biomedical analysis, 2(2), 281-7. https://doi.org/10.1016/0731-7085(84)80078-3
Wideman, J, et al. "Characterization of small amounts of peptides and proteins." Journal of pharmaceutical and biomedical analysis vol. 2,2 (1984): 281-7. doi: https://doi.org/10.1016/0731-7085(84)80078-3
Wideman J, Pan YC, Lahm HW, Chang M, Stein S. Characterization of small amounts of peptides and proteins. J Pharm Biomed Anal. 1984;2(2):281-7. doi: 10.1016/0731-7085(84)80078-3. PMID: 16867755.
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J Pharm Biomed Anal. 1984;2(2):281-7. doi: 10.1016/0731-7085(84)80078-3.

Characterization of small amounts of peptides and proteins.

Journal of pharmaceutical and biomedical analysis

J Wideman, Y C Pan, H W Lahm, M Chang, S Stein

Affiliations

  1. Biopolymer Research Department, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA.

PMID: 16867755 DOI: 10.1016/0731-7085(84)80078-3

Abstract

The use of microchemical methods for the characterization of both natural and recombinant proteins of biomedical importance is discussed. The methods include gel electrophoresis, high-performance liquid chromatography, protein fragmentation, amino acid analysis and automated Edman degradation. Each procedure is applicable at the picomole level.

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