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Reynolds DL, Repta AJ, Sternson LA. Cation exchange contribution to the retention of specific quaternary ammonium compounds in reversed-phase high-performance liquid chromatography. J Pharm Biomed Anal. 1983;1(3):339-46doi: 10.1016/0731-7085(83)80046-6.
Reynolds, D. L., Repta, A. J., & Sternson, L. A. (1983). Cation exchange contribution to the retention of specific quaternary ammonium compounds in reversed-phase high-performance liquid chromatography. Journal of pharmaceutical and biomedical analysis, 1(3), 339-46. https://doi.org/10.1016/0731-7085(83)80046-6
Reynolds, D L, et al. "Cation exchange contribution to the retention of specific quaternary ammonium compounds in reversed-phase high-performance liquid chromatography." Journal of pharmaceutical and biomedical analysis vol. 1,3 (1983): 339-46. doi: https://doi.org/10.1016/0731-7085(83)80046-6
Reynolds DL, Repta AJ, Sternson LA. Cation exchange contribution to the retention of specific quaternary ammonium compounds in reversed-phase high-performance liquid chromatography. J Pharm Biomed Anal. 1983;1(3):339-46. doi: 10.1016/0731-7085(83)80046-6. PMID: 16867804.
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J Pharm Biomed Anal. 1983;1(3):339-46. doi: 10.1016/0731-7085(83)80046-6.

Cation exchange contribution to the retention of specific quaternary ammonium compounds in reversed-phase high-performance liquid chromatography.

Journal of pharmaceutical and biomedical analysis

D L Reynolds, A J Repta, L A Sternson

Affiliations

  1. Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, KS 66045, USA.

PMID: 16867804 DOI: 10.1016/0731-7085(83)80046-6

Abstract

The influence of electrolytes on the retention of organic cationic solutes in reversed-phase high-performance liquid chromatography (RP-HPLC) has been investigated. The effects of the nature and concentration of electrolytes and mobile phase pH on the retention of two model quaternary ammonium compounds were studied on mu-Bondapak C18 stationary phase with aqueous methanolic eluents. The nature and concentration of inorganic cations added to the mobile phase modified the retention of the solutes. The counter anion of the added electrolyte did not perceptibly influence solute retention at constant mobile phase pH, although it did significantly influence solute retention when the electrolytes were added to unbuffered mobile phases. The retention data are consistent with the inclusion of an ion exchange contribution to the retention of cationic solutes in the systems investigated.

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