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Holthuis JJ, Römkens FM, Pinedo HM, et al. Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with electrochemical detection. J Pharm Biomed Anal. 1983;1(1):89-97doi: 10.1016/0731-7085(83)80012-0.
Holthuis, J. J., Römkens, F. M., Pinedo, H. M., & van Oort, W. J. (1983). Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with electrochemical detection. Journal of pharmaceutical and biomedical analysis, 1(1), 89-97. https://doi.org/10.1016/0731-7085(83)80012-0
Holthuis, J J, et al. "Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with electrochemical detection." Journal of pharmaceutical and biomedical analysis vol. 1,1 (1983): 89-97. doi: https://doi.org/10.1016/0731-7085(83)80012-0
Holthuis JJ, Römkens FM, Pinedo HM, van Oort WJ. Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with electrochemical detection. J Pharm Biomed Anal. 1983;1(1):89-97. doi: 10.1016/0731-7085(83)80012-0. PMID: 16867838.
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J Pharm Biomed Anal. 1983;1(1):89-97. doi: 10.1016/0731-7085(83)80012-0.

Plasma assay for the antineoplastic agent VP 16-213 (etoposide) using high-performance liquid chromatography with electrochemical detection.

Journal of pharmaceutical and biomedical analysis

J J Holthuis, F M Römkens, H M Pinedo, W J van Oort

Affiliations

  1. Pharmaceutical Laboratory, State University of Utrecht, Catharijnesingel 60, 3511 GH Utrecht, The Netherlands.

PMID: 16867838 DOI: 10.1016/0731-7085(83)80012-0

Abstract

A sensitive high-performance liquid chromatographic (HPLC) assay of the antineoplastic agent VP 16-213 (etoposide) in plasma is described. The system discriminates between the parent compound and possible metabolites, including the aglycone and the cis isomer. After extraction with 1,2-dichloroethane the drugs are chromatographed on a reversed-phase phenyl column with amperometric detection. Quantitative response is linear up to 250 ng/ml for 1 ml human plasma and up to 40.0 mug/ml for 0.1 ml human plasma. The detection limit is ca 2 ng/ml in plasma. Preliminary pharmacokinetic results show that the sensitivity and selectivity of the assay are adequate to establish plasma concentrations over 8-12 half-lives during elimination of the drug.

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