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Kang XF, Cheley S, Rice-Ficht AC, et al. A storable encapsulated bilayer chip containing a single protein nanopore. J Am Chem Soc. 2007;129(15):4701-5doi: 10.1021/ja068654g.
Kang, X. F., Cheley, S., Rice-Ficht, A. C., & Bayley, H. (2007). A storable encapsulated bilayer chip containing a single protein nanopore. Journal of the American Chemical Society, 129(15), 4701-5. https://doi.org/10.1021/ja068654g
Kang, Xiao-feng, et al. "A storable encapsulated bilayer chip containing a single protein nanopore." Journal of the American Chemical Society vol. 129,15 (2007): 4701-5. doi: https://doi.org/10.1021/ja068654g
Kang XF, Cheley S, Rice-Ficht AC, Bayley H. A storable encapsulated bilayer chip containing a single protein nanopore. J Am Chem Soc. 2007 Apr 18;129(15):4701-5. doi: 10.1021/ja068654g. Epub 2007 Mar 22. PMID: 17375923.
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J Am Chem Soc. 2007 Apr 18;129(15):4701-5. doi: 10.1021/ja068654g. Epub 2007 Mar 22.

A storable encapsulated bilayer chip containing a single protein nanopore.

Journal of the American Chemical Society

Xiao-feng Kang, Stephen Cheley, Allison C Rice-Ficht, Hagan Bayley

Affiliations

  1. Department of Molecular and Cellular Medicine, The Texas A&M University System Health Science Center, College Station, TX 77843-1114, USA.

PMID: 17375923 DOI: 10.1021/ja068654g

Abstract

A robust, portable chip containing a single protein nanopore would be a significant development in the practical application of stochastic sensing technology. Here, we describe a chip in which a single alpha-hemolysin (alphaHL) pore in a planar phospholipid bilayer is sandwiched between two layers of agarose gel. These encapsulated nanopore chips remain functional after storage for weeks. The detection of the second messenger inositol 1,4,5-trisphosphate (IP3) was demonstrated with a chip containing a genetically engineered alphaHL pore as the sensor element.

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