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Osaka E, Suzuki T, Osaka S, et al. Survivin as a prognostic factor for osteosarcoma patients. Acta Histochem Cytochem. 2006;39(3):95-100doi: 10.1267/ahc.06005.
Osaka, E., Suzuki, T., Osaka, S., Yoshida, Y., Sugita, H., Asami, S., Tabata, K., Hemmi, A., Sugitani, M., Nemoto, N., & Ryu, J. (2006). Survivin as a prognostic factor for osteosarcoma patients. Acta histochemica et cytochemica, 39(3), 95-100. https://doi.org/10.1267/ahc.06005
Osaka, Eiji, et al. "Survivin as a prognostic factor for osteosarcoma patients." Acta histochemica et cytochemica vol. 39,3 (2006): 95-100. doi: https://doi.org/10.1267/ahc.06005
Osaka E, Suzuki T, Osaka S, Yoshida Y, Sugita H, Asami S, Tabata K, Hemmi A, Sugitani M, Nemoto N, Ryu J. Survivin as a prognostic factor for osteosarcoma patients. Acta Histochem Cytochem. 2006 Jul 01;39(3):95-100. doi: 10.1267/ahc.06005. Epub 2006 May 26. PMID: 17327929; PMCID: PMC1790971.
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Acta Histochem Cytochem. 2006 Jul 01;39(3):95-100. doi: 10.1267/ahc.06005. Epub 2006 May 26.

Survivin as a prognostic factor for osteosarcoma patients.

Acta histochemica et cytochemica

Eiji Osaka, Takashi Suzuki, Shunzo Osaka, Yukihiro Yoshida, Hideyuki Sugita, Satoru Asami, Keiichi Tabata, Akihiro Hemmi, Masahiko Sugitani, Norimichi Nemoto, Junnosuke Ryu

Affiliations

  1. Department of Orthopaedic Surgery, Nihon University School of Medicine, 30-1 Oyaguchikamimachi, Itabashi-ku, Tokyo 173-8610, Japan. [email protected]

PMID: 17327929 PMCID: PMC1790971 DOI: 10.1267/ahc.06005

Abstract

PURPOSE: Survivin is one of the apoptosis inhibitor genes and is rarely expressed in adult -tissues. However, survivin expression has been detected in various human cancers and -correlations have been recognized between the level of expression of this gene in tumors and prognosis. In this study, we investigated the correlations between survivin mRNA expression in osteosarcoma tissues and clinicopathological parameters.

METHODS: There were 22 osteosarcoma patients in our hospital with paraffin-embedded -tissues which could be extracted from biopsy specimens. We used the RT-PCR method after extracting total RNA and conducted a densitometric analysis to determine the ratio of survivin relative to h-GAPDH as an internal marker.

RESULTS: Expression of survivin mRNA was detected in all osteosarcoma samples. Patients with metastasis had high survivin mRNA levels in initial biopsy specimens (p<0.01). Moreover, there was a statistically significant difference in survivin mRNA expression between -patients with and without metastasis (p<0.01).

CONCLUSION: We concluded that high levels of survivin mRNA expression suggest poor prognosis for osteosarcoma patients.

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