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de Oliveira JP, Fernandes F, Cruz AK, et al. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biol Dis. 2007;6:5doi: 10.1186/1475-9292-6-5.
de Oliveira, J. P., Fernandes, F., Cruz, A. K., Trombela, V., Monteiro, E., Camargo, A. A., Barral, A., & de Oliveira, C. I. (2007). Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid biology and disease, 65. https://doi.org/10.1186/1475-9292-6-5
de Oliveira, João Paulo C, et al. "Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping." Kinetoplastid biology and disease vol. 6 (2007): 5. doi: https://doi.org/10.1186/1475-9292-6-5
de Oliveira JP, Fernandes F, Cruz AK, Trombela V, Monteiro E, Camargo AA, Barral A, de Oliveira CI. Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping. Kinetoplastid Biol Dis. 2007 Jun 21;6:5. doi: 10.1186/1475-9292-6-5. PMID: 17584940; PMCID: PMC1919383.
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Kinetoplastid Biol Dis. 2007 Jun 21;6:5. doi: 10.1186/1475-9292-6-5.

Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping.

Kinetoplastid biology and disease

João Paulo C de Oliveira, Flora Fernandes, Angela K Cruz, Viviane Trombela, Elisângela Monteiro, Anamaria A Camargo, Aldina Barral, Camila I de Oliveira

Affiliations

  1. Centro de Pesquisas Gonçalo Moniz, FIOCRUZ, Rua Waldemar Falcão, 121, 40296-710, Salvador, BA, Brazil. [email protected]

PMID: 17584940 PMCID: PMC1919383 DOI: 10.1186/1475-9292-6-5

Abstract

BACKGROUND: Leishmania (Leishmania) amazonensis infection in man results in a clinical spectrum of disease manifestations ranging from cutaneous to mucosal or visceral involvement. In the present study, we have investigated the genetic variability of 18 L. amazonensis strains isolated in northeastern Brazil from patients with different clinical manifestations of leishmaniasis. Parasite DNA was analyzed by sequencing of the ITS flanking the 5.8 S subunit of the ribosomal RNA genes, by RAPD and SSR-PCR and by PFGE followed by hybridization with gene-specific probes.

RESULTS: ITS sequencing and PCR-based methods revealed genetic heterogeneity among the L. amazonensis isolates examined and molecular karyotyping also showed variation in the chromosome size of different isolates. Unrooted genetic trees separated strains into different groups.

CONCLUSION: These results indicate that L. amazonensis strains isolated from leishmaniasis patients from northeastern Brazil are genetically diverse, however, no correlation between genetic polymorphism and phenotype were found.

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