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von Helden G, Wyttenbach T, Bowers MT. Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography. Science. 1995;267(5203):1483-5doi: 10.1126/science.267.5203.1483.
von Helden, G., Wyttenbach, T., & Bowers, M. T. (1995). Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography. Science (New York, N.Y.), 267(5203), 1483-5. https://doi.org/10.1126/science.267.5203.1483
von Helden, G, et al. "Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography." Science (New York, N.Y.) vol. 267,5203 (1995): 1483-5. doi: https://doi.org/10.1126/science.267.5203.1483
von Helden G, Wyttenbach T, Bowers MT. Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography. Science. 1995 Mar 10;267(5203):1483-5. doi: 10.1126/science.267.5203.1483. PMID: 17743549.
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Science. 1995 Mar 10;267(5203):1483-5. doi: 10.1126/science.267.5203.1483.

Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography.

Science (New York, N.Y.)

G von Helden, T Wyttenbach, M T Bowers

PMID: 17743549 DOI: 10.1126/science.267.5203.1483

Abstract

Conformational data for macromolecules in the gas phase have been obtained by the coupling of a matrix-assisted laser desorption ion source to an ion chromatograph. A series of polyethylene glycol (PEG) polymers "cationized" (converted to a cation) by sodium ions (Na(+)PEG9 to Na(+)PEG19) and a protonated neurotransmitter protein, bradykinin, were studied. Mobilities of Na(+)PEG9 to Na(+)PEG19 are reported. Detailed modeling of Na(+)PEG9 with molecular mechanics methods indicates that the lowest energy structure has the Na(+) ion "solvated" by the polymer chain with seven oxygen atoms as nearest neighbors. The agreement between the model and experiment is within 1 percent for Na(+)PEG9, Na(+)PEG13, and Na(+)PEG17, giving strong support to both the method and the deduced structures. Similar agreement was obtained in initial studies that modeled experimental data for arginine-protonated bradykinin.

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