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Shah DM, Horsch RB, Klee HJ, et al. Engineering herbicide tolerance in transgenic plants. Science. 1986;233(4762):478-81doi: 10.1126/science.233.4762.478.
Shah, D. M., Horsch, R. B., Klee, H. J., Kishore, G. M., Winter, J. A., Tumer, N. E., Hironaka, C. M., Sanders, P. R., Gasser, C. S., Aykent, S., Siegel, N. R., Rogers, S. G., & Fraley, R. T. (1986). Engineering herbicide tolerance in transgenic plants. Science (New York, N.Y.), 233(4762), 478-81. https://doi.org/10.1126/science.233.4762.478
Shah, D M, et al. "Engineering herbicide tolerance in transgenic plants." Science (New York, N.Y.) vol. 233,4762 (1986): 478-81. doi: https://doi.org/10.1126/science.233.4762.478
Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Tumer NE, Hironaka CM, Sanders PR, Gasser CS, Aykent S, Siegel NR, Rogers SG, Fraley RT. Engineering herbicide tolerance in transgenic plants. Science. 1986 Jul 25;233(4762):478-81. doi: 10.1126/science.233.4762.478. PMID: 17794571.
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Science. 1986 Jul 25;233(4762):478-81. doi: 10.1126/science.233.4762.478.

Engineering herbicide tolerance in transgenic plants.

Science (New York, N.Y.)

D M Shah, R B Horsch, H J Klee, G M Kishore, J A Winter, N E Tumer, C M Hironaka, P R Sanders, C S Gasser, S Aykent, N R Siegel, S G Rogers, R T Fraley

PMID: 17794571 DOI: 10.1126/science.233.4762.478

Abstract

The herbicide glyphosate is a potent inhibitor of the enzyme 5-enolpyruvylshikimate- 3-phosphate (EPSP) synthase in higher plants. A complementary DNA (cDNA) clone encoding EPSP synthase was isolated from a complementary DNA library of a glyphosate-tolerant Petunia hybrida cell line (MP4-G) that overproduces the enzyme. This cell line was shown to overproduce EPSP synthase messenger RNA as a result of a 20-fold amplification of the gene. A chimeric EPSP synthase gene was constructed with the use of the cauliflower mosaic virus 35S promoter to attain high level expression of EPSP synthase and introduced into petunia cells. Transformed petunia cells as well as regenerated transgenic plants were tolerant to glyphosate.

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