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Chino H, Sakurai S, Ohtaki T, et al. Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro. Science. 1974;183(4124):529-30doi: 10.1126/science.183.4124.529.
Chino, H., Sakurai, S., Ohtaki, T., Ikekawa, N., Miyazaki, H., Ishibashi, M., & Abuki, H. (1974). Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro. Science (New York, N.Y.), 183(4124), 529-30. https://doi.org/10.1126/science.183.4124.529
Chino, H, et al. "Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro." Science (New York, N.Y.) vol. 183,4124 (1974): 529-30. doi: https://doi.org/10.1126/science.183.4124.529
Chino H, Sakurai S, Ohtaki T, Ikekawa N, Miyazaki H, Ishibashi M, Abuki H. Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro. Science. 1974 Feb 08;183(4124):529-30. doi: 10.1126/science.183.4124.529. PMID: 17773042.
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Science. 1974 Feb 08;183(4124):529-30. doi: 10.1126/science.183.4124.529.

Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro.

Science (New York, N.Y.)

H Chino, S Sakurai, T Ohtaki, N Ikekawa, H Miyazaki, M Ishibashi, H Abuki

PMID: 17773042 DOI: 10.1126/science.183.4124.529

Abstract

An in vitro study in which isolated prothoracic glands of the Bombyx silkworm were cultured has provided definite evidence that the prothoracic gland is the site where molting hormone is synthesized. The hormone behaved very similarly to free ecdysone on thin-layer chromatography. Analysis by liquid chromatography and mass fragmentography revealed that the hormone is identical with alpha-ecdysone.

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