Display options
Cite
Patrick DM, Petric M, Skowronski DM, et al. An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. Can J Infect Dis Med Microbiol. 2006;17(6):330-6doi: 10.1155/2006/152612.
Patrick, D. M., Petric, M., Skowronski, D. M., Guasparini, R., Booth, T. F., Krajden, M., McGeer, P., Bastien, N., Gustafson, L., Dubord, J., Macdonald, D., David, S. T., Srour, L. F., Parker, R., Andonov, A., Isaac-Renton, J., Loewen, N., McNabb, G., McNabb, A., Goh, S. H., Henwick, S., Astell, C., Guo, J. P., Drebot, M., Tellier, R., Plummer, F., & Brunham, R. C. (2006). An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale, 17(6), 330-6. https://doi.org/10.1155/2006/152612
Patrick, David M, et al. "An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus." The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale vol. 17,6 (2006): 330-6. doi: https://doi.org/10.1155/2006/152612
Patrick DM, Petric M, Skowronski DM, Guasparini R, Booth TF, Krajden M, McGeer P, Bastien N, Gustafson L, Dubord J, Macdonald D, David ST, Srour LF, Parker R, Andonov A, Isaac-Renton J, Loewen N, McNabb G, McNabb A, Goh SH, Henwick S, Astell C, Guo JP, Drebot M, Tellier R, Plummer F, Brunham RC. An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus. Can J Infect Dis Med Microbiol. 2006 Nov;17(6):330-6. doi: 10.1155/2006/152612. PMID: 18382647; PMCID: PMC2095096.
Copy Download .nbib Format: NLM
Share it on

Can J Infect Dis Med Microbiol. 2006 Nov;17(6):330-6. doi: 10.1155/2006/152612.

An Outbreak of Human Coronavirus OC43 Infection and Serological Cross-reactivity with SARS Coronavirus.

The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale

David M Patrick, Martin Petric, Danuta M Skowronski, Roland Guasparini, Timothy F Booth, Mel Krajden, Patrick McGeer, Nathalie Bastien, Larry Gustafson, Janet Dubord, Diane Macdonald, Samara T David, Leila F Srour, Robert Parker, Anton Andonov, Judith Isaac-Renton, Nadine Loewen, Gail McNabb, Alan McNabb, Swee-Han Goh, Scott Henwick, Caroline Astell, Jian Ping Guo, Michael Drebot, Raymond Tellier, Francis Plummer, Robert C Brunham

Affiliations

  1. University of British Columbia Centre for Disease Control.

PMID: 18382647 PMCID: PMC2095096 DOI: 10.1155/2006/152612

Abstract

BACKGROUND: In summer 2003, a respiratory outbreak was investigated in British Columbia, during which nucleic acid tests and serology unexpectedly indicated reactivity for severe acute respiratory syndrome coronavirus (SARS-CoV).

METHODS: Cases at a care facility were epidemiologically characterized and sequentially investigated for conventional agents of respiratory infection, SARS-CoV and other human CoVs. Serological cross-reactivity between SARS-CoV and human CoV-OC43 (HCoV-OC43) was investigated by peptide spot assay.

RESULTS: Ninety-five of 142 residents (67%) and 53 of 160 staff members (33%) experienced symptoms of respiratory infection. Symptomatic residents experienced cough (66%), fever (21%) and pneumonia (12%). Eight residents died, six with pneumonia. No staff members developed pneumonia. Findings on reverse transcriptase-polymerase chain reaction assays for SARS-CoV at a national reference laboratory were suspected to represent false positives, but this was confounded by concurrent identification of antibody to N protein on serology. Subsequent testing by reverse transcriptase-polymerase chain reaction confirmed HCoV-OC43 infection. Convalescent serology ruled out SARS. Notably, sera demonstrated cross-reactivity against nucleocapsid peptide sequences common to HCoV-OC43 and SARS-CoV.

CONCLUSIONS: These findings underscore the virulence of human CoV-OC43 in elderly populations and confirm that cross-reactivity to antibody against nucleocapsid proteins from these viruses must be considered when interpreting serological tests for SARS-CoV.

Keywords: Coronavirus; Human coronavirus OC43; Outbreak; Respiratory infection; SARS coronavirus; SARS-CoV; Severe acute respiratory syndrome

References

  1. Emerg Infect Dis. 2006 Jan;12(1):155-8 - PubMed
  2. Epidemiol Infect. 2005 Apr;133(2):273-7 - PubMed
  3. Science. 2003 Jun 20;300(5627):1961-6 - PubMed
  4. Clin Infect Dis. 2000 Jul;31(1):96-100 - PubMed
  5. N Engl J Med. 2003 May 15;348(20):1995-2005 - PubMed
  6. Diagn Microbiol Infect Dis. 2005 Sep;53(1):47-55 - PubMed
  7. Med J Aust. 2003 Jun 2;178(11):555-8 - PubMed
  8. Lancet. 2003 Oct 25;362(9393):1353-8 - PubMed
  9. Nature. 2003 May 15;423(6937):240 - PubMed
  10. CMAJ. 2003 Aug 19;169(4):285-92 - PubMed
  11. DNA Cell Biol. 2005 Aug;24(8):521-7 - PubMed
  12. Virology. 2004 Jul 1;324(2):251-6 - PubMed
  13. Virus Res. 2003 May;93(1):51-62 - PubMed
  14. N Engl J Med. 2003 May 15;348(20):1953-66 - PubMed
  15. N Engl J Med. 2003 Jul 24;349(4):319-20 - PubMed
  16. MMWR Morb Mortal Wkly Rep. 2003 Oct 17;52(41):986-7 - PubMed
  17. N Engl J Med. 2003 May 15;348(20):1967-76 - PubMed
  18. J Virol Methods. 2004 Dec 1;122(1):29-36 - PubMed
  19. J Infect Dis. 2005 Jun 15;191(12):2033-7 - PubMed
  20. Clin Vaccine Immunol. 2006 Mar;13(3):409-14 - PubMed
  21. Virus Res. 1989 Jan;12(1):1-9 - PubMed
  22. Clin Infect Dis. 2003 Apr 15;36(8):985-9 - PubMed
  23. Clin Diagn Lab Immunol. 2005 Nov;12(11):1317-21 - PubMed
  24. N Engl J Med. 2003 Dec 18;349(25):2431-41 - PubMed
  25. Biochem Biophys Res Commun. 2005 Dec 23;338(3):1654-60 - PubMed
  26. Science. 2003 Oct 31;302(5646):767 - PubMed
  27. Science. 2003 May 30;300(5624):1399-404 - PubMed
  28. Lancet. 2003 Apr 19;361(9366):1319-25 - PubMed

Publication Types