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Nag MK, Deshpande YG, Beck D, et al. The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells. Mediators Inflamm. 1995;4(2):90-4doi: 10.1155/S0962935195000147.
Nag, M. K., Deshpande, Y. G., Beck, D., Li, A., & Kaminski, D. L. (1995). The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells. Mediators of inflammation, 4(2), 90-4. https://doi.org/10.1155/S0962935195000147
Nag, M K, et al. "The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells." Mediators of inflammation vol. 4,2 (1995): 90-4. doi: https://doi.org/10.1155/S0962935195000147
Nag MK, Deshpande YG, Beck D, Li A, Kaminski DL. The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells. Mediators Inflamm. 1995;4(2):90-4. doi: 10.1155/S0962935195000147. PMID: 18475621; PMCID: PMC2365627.
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Mediators Inflamm. 1995;4(2):90-4. doi: 10.1155/S0962935195000147.

The effect of lysolecithin on prostanoid and platelet-activating factor formation by human gall-bladder mucosal cells.

Mediators of inflammation

M K Nag, Y G Deshpande, D Beck, A Li, D L Kaminski

Affiliations

  1. Department of Surgery St Louis University Health Sciences Center 3635 Vista Ave. at Grand Blvd P.O. Box 15250 St Louis MO 63110-0250 USA.

PMID: 18475621 PMCID: PMC2365627 DOI: 10.1155/S0962935195000147

Abstract

It has been demonstrated that lysolecithin (lysophosphatidyl choline, LPC) produces experimental cholecystitis in cats mediated by arachidonic acid metabolites. LPC is a cytolytic agent that has been postulated as a contributing factor in the development of cholecystitis in humans. The purpose of this research was to evaluate the effect of LPC on human gall-bladder mucosal cell phospholipase A(2) and cyclooxygenase activity. Gall-bladder mucosal cells were isolated from the gall-bladders of patients undergoing routine cholecystectomy. Fresh, isolated cells were maintained in tissue culture and stimulated with varying doses of LPC. Platelet-activating factor concentration was quantitated as an index of phospholipase A(2) activity and prostanoids were measured as an index of cyclooxygenase activity. Also, the effect of LPC on cyclooxygenase 1 and 2 expression in microsomal protein was evaluated. LPC caused dose related increases in 6-keto-PGF(1alpha) and PAF produced by human gall-bladder mucosal cells. Exposure of human gall-bladder mucosal cells to LPC failed to elicit expression of constitutive cyclooxygenase-1, while the expression of inducible cyclooxygenase-2 was increased. The results of this study indicate that LPC induces the formation of prostanoids and PAF by human gall-bladder mucosal cells, suggesting that this substance may promote the development of gall-bladder inflammation.

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