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Heine RP, Harding S, Emmett P, et al. In vitro bacterial contamination of amniotic fluid: effects on fluorescence polarization lung maturity testing. Infect Dis Obstet Gynecol. 1995;3(3):98-101doi: 10.1155/S1064744995000408.
Heine, R. P., Harding, S., Emmett, P., Ashwood, E., & Lenke, R. R. (1995). In vitro bacterial contamination of amniotic fluid: effects on fluorescence polarization lung maturity testing. Infectious diseases in obstetrics and gynecology, 3(3), 98-101. https://doi.org/10.1155/S1064744995000408
Heine, R P, et al. "In vitro bacterial contamination of amniotic fluid: effects on fluorescence polarization lung maturity testing." Infectious diseases in obstetrics and gynecology vol. 3,3 (1995): 98-101. doi: https://doi.org/10.1155/S1064744995000408
Heine RP, Harding S, Emmett P, Ashwood E, Lenke RR. In vitro bacterial contamination of amniotic fluid: effects on fluorescence polarization lung maturity testing. Infect Dis Obstet Gynecol. 1995;3(3):98-101. doi: 10.1155/S1064744995000408. PMID: 18476029; PMCID: PMC2364429.
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Infect Dis Obstet Gynecol. 1995;3(3):98-101. doi: 10.1155/S1064744995000408.

In vitro bacterial contamination of amniotic fluid: effects on fluorescence polarization lung maturity testing.

Infectious diseases in obstetrics and gynecology

R P Heine, S Harding, P Emmett, E Ashwood, R R Lenke

Affiliations

  1. Department of Obstetrics and Gynecology University of Colorado, Denver, CO, USA.

PMID: 18476029 PMCID: PMC2364429 DOI: 10.1155/S1064744995000408

Abstract

OBJECTIVE: We sought to determine the effect of bacteria on fluorescence polarization (FPOL) testing of amniotic fluid.

METHODS: Fusobacterium necrophorum and Escherichia coli were inoculated at concentrations of 10(3) and 10(6)/ml in amniotic-fluid specimens from 4 patients with no clinical or laboratory evidence of infection. The FPOL results were obtained at inoculation and again at 24 h of incubation. The results were compared using analysis of variance (ANOVA).

RESULTS: The FPOL results from inoculated specimens were all within 2% of the uninoculated controls. The specimens incubated with bacteria showed a < 1-19% variation when compared with the time-zero uninoculated controls. However, uninoculated controls incubated for 24 h exhibited a 2-12% variation when compared with the time-zero controls, suggesting that the variation present was not secondary to the bacterial co-incubation.

CONCLUSIONS: In vitro, neither bacterial inoculation nor prolonged co-incubation influences FPOL results beyond the effect of incubation alone. FPOL appears to be an appropriate test to assess fetal lung maturity in patients in whom intraamniotic infection is a concern.

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