Display options
Cite
Nino-Soto MI, Jozani RJ, Bridle B, et al. Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs. BMC Res Notes. 2008;1:31doi: 10.1186/1756-0500-1-31.
Nino-Soto, M. I., Jozani, R. J., Bridle, B., & Mallard, B. A. (2008). Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs. BMC research notes, 131. https://doi.org/10.1186/1756-0500-1-31
Nino-Soto, Maria I, et al. "Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs." BMC research notes vol. 1 (2008): 31. doi: https://doi.org/10.1186/1756-0500-1-31
Nino-Soto MI, Jozani RJ, Bridle B, Mallard BA. Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs. BMC Res Notes. 2008 Jun 23;1:31. doi: 10.1186/1756-0500-1-31. PMID: 18710484; PMCID: PMC2529311.
Copy Download .nbib Format: NLM
Share it on

BMC Res Notes. 2008 Jun 23;1:31. doi: 10.1186/1756-0500-1-31.

Analysis of gene expression patterns by microarray hybridization in blood mononuclear cells of SLA-DRB1 defined Canadian Yorkshire pigs.

BMC research notes

Maria I Nino-Soto, Razi Jafari Jozani, Byram Bridle, Bonnie A Mallard

Affiliations

  1. Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ont, N1G 2W1, Canada. [email protected]

PMID: 18710484 PMCID: PMC2529311 DOI: 10.1186/1756-0500-1-31

Abstract

BACKGROUND: The Swine Leukocyte Antigen (SLA) system encodes molecules for self-nonself discrimination and is associated with immune responses and disease resistance. Three lines of pigs defined by their SLA-DRB1 alleles were developed at the University of Guelph for xenotransplantation and immune response studies. The aim of this project was to explore the potential association between defined SLA-DRB1 alleles and gene transcriptional patterns of other immune-related genes in blood mononuclear cells.

FINDINGS: Three SLA-DRB1 alleles were characterized using a RT-PCR-based sequencing method. The loci represented included a new allele, DRB1*04ns01. Next, microarray heterologous (bovine-porcine) hybridization together with qPCR were used to explore differential gene expression between SLA-DRB1-defined groups. Microarray analysis showed significant (p < 0.01) differential expression for 5 genes, mostly related to inflammation. Genes varied according to the comparison analyzed. Further testing with qPCR revealed the same trend of differential expression for 4 of the genes, although statistical significance was reached for only one.

CONCLUSION: A new SLA-DRB1 allele was characterized. A potential association was found between SLA-DRB1 alleles and inflammation-related genes. However, the influence of other genes cannot be ruled out. These preliminary findings agree with other studies linking MHC haplotypes and inflammation processes, including autoimmune disease. The study provides an initial view of the biological interactions between the SLA complex and other immune-related genes. Future studies will focus on characterization of SLA-haplotypes associated with these particular alleles and the dynamics of the immune response to antigenic challenges.

References

  1. Immunogenetics. 2006 Nov;58(11):873-82 - PubMed
  2. Tissue Antigens. 2004 Dec;64(6):631-49 - PubMed
  3. Rev Sci Tech. 1998 Apr;17(1):95-107 - PubMed
  4. Nucleic Acids Res. 2002 Feb 15;30(4):e15 - PubMed
  5. Curr Opin Immunol. 2005 Oct;17(5):498-504 - PubMed
  6. Genes Immun. 2006 Sep;7(6):450-67 - PubMed
  7. Tissue Antigens. 2005 Dec;66(6):623-39 - PubMed
  8. J Exp Biol. 2007 May;210(Pt 9):1602-6 - PubMed
  9. Nat Genet. 2001 Dec;29(4):365-71 - PubMed
  10. Annu Rev Immunol. 2003;21:629-57 - PubMed
  11. Int J Biol Sci. 2007 Feb 10;3(3):179-84 - PubMed
  12. Can J Vet Res. 1993 Jan;57(1):14-8 - PubMed
  13. Exp Parasitol. 1993 Aug;77(1):23-35 - PubMed
  14. Transpl Immunol. 2007 Jun;17(4):262-70 - PubMed
  15. Xenotransplantation. 2006 Jul;13(4):299-307 - PubMed
  16. Vet Immunol Immunopathol. 2004 Sep;101(1-2):1-17 - PubMed
  17. Reprod Nutr Dev. 1989;29(5):569-76 - PubMed
  18. Hum Immunol. 1999 Apr;60(4):312-22 - PubMed
  19. Nucleic Acids Res. 1994 Nov 11;22(22):4673-80 - PubMed
  20. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
  21. J Anim Sci. 1991 Feb;69(2):559-64 - PubMed

Publication Types