Display options
Cite
Clawson ML, Heaton MP, Keele JW, et al. A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle. BMC Res Notes. 2008;1:32doi: 10.1186/1756-0500-1-32.
Clawson, M. L., Heaton, M. P., Keele, J. W., Smith, T. P., Harhay, G. P., Richt, J. A., & Laegreid, W. W. (2008). A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle. BMC research notes, 132. https://doi.org/10.1186/1756-0500-1-32
Clawson, Michael L, et al. "A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle." BMC research notes vol. 1 (2008): 32. doi: https://doi.org/10.1186/1756-0500-1-32
Clawson ML, Heaton MP, Keele JW, Smith TP, Harhay GP, Richt JA, Laegreid WW. A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle. BMC Res Notes. 2008 Jun 23;1:32. doi: 10.1186/1756-0500-1-32. PMID: 18710485; PMCID: PMC2525647.
Copy Download .nbib Format: NLM
Share it on

BMC Res Notes. 2008 Jun 23;1:32. doi: 10.1186/1756-0500-1-32.

A sequencing strategy for identifying variation throughout the prion gene of BSE-affected cattle.

BMC research notes

Michael L Clawson, Michael P Heaton, John W Keele, Timothy Pl Smith, Gregory P Harhay, Juergen A Richt, William W Laegreid

Affiliations

  1. United States Department of Agriculture (USDA), Agricultural Research Service (ARS), US Meat Animal Research Center (USMARC), Clay Center, NE 68933, USA. [email protected]

PMID: 18710485 PMCID: PMC2525647 DOI: 10.1186/1756-0500-1-32

Abstract

BACKGROUND: Classical and atypical bovine spongiform encephalopathies (BSEs) are cattle prion diseases. Distinct bovine prion gene (PRNP) alleles have been associated with classical and atypical BSE susceptibility. However, the full extent of PRNP allele association with BSE susceptibility is not known. A systematic sequence-based genotyping method that detects variation throughout PRNP would be useful for: 1) detecting rare PRNP alleles that may be present in BSE-affected animals and 2) testing PRNP alleles for an association with either classical or atypical BSE susceptibility.

FINDINGS: We improved a Sanger-based sequencing strategy for detecting bovine PRNP variation through all exons, introns, and part of the promoter (25.2 kb). Our current method can detect 389 known and other potentially unknown PRNP polymorphisms that may be present in BSE-affected cattle. We determined PRNP genotypes for the first U.S. BSE case and her sire. Previously unknown PRNP polymorphisms were not detected in either animal and all PRNP genotypes support the sire-daughter relationship.

CONCLUSION: The methodologies described here characterize variation throughout PRNP. Consequently, rare PRNP alleles that may be present in BSE-affected cattle can be detected.

References

  1. Mamm Genome. 2001 Mar;12(3):219-26 - PubMed
  2. Am J Hum Genet. 2005 Mar;76(3):449-62 - PubMed
  3. BMC Vet Res. 2008 Jul 14;4:25 - PubMed
  4. Am J Hum Genet. 2001 Apr;68(4):978-89 - PubMed
  5. Hum Genet. 2005 Nov;118(2):166-74 - PubMed
  6. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13363-83 - PubMed
  7. PLoS One. 2008 Mar 19;3(3):e1830 - PubMed
  8. Vet Rec. 1987 Oct 31;121(18):419-20 - PubMed
  9. Mamm Genome. 2003 Nov;14(11):765-77 - PubMed
  10. Neurogenetics. 2004 Feb;5(1):19-25 - PubMed
  11. Vet Res. 2008 Jul-Aug;39(4):15 - PubMed
  12. BMC Biol. 2006 Oct 02;4:33 - PubMed
  13. BMC Genet. 2006 Nov 08;7:51 - PubMed
  14. Vet Rec. 1991 Mar 2;128(9):199-203 - PubMed
  15. Nat Genet. 2006 Mar;38(3):375-81 - PubMed
  16. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3065-70 - PubMed
  17. BMC Genet. 2007 Apr 16;8:15 - PubMed
  18. EMBO Rep. 2004 Jan;5(1):110-5 - PubMed

Publication Types