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Drews SJ, Eshaghi A, Pyskir D, et al. The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens. Diagn Pathol. 2008;3:37doi: 10.1186/1746-1596-3-37.
Drews, S. J., Eshaghi, A., Pyskir, D., Chedore, P., Lombos, E., Broukhanski, G., Higgins, R., Fisman, D. N., Blair, J., & Jamieson, F. (2008). The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens. Diagnostic pathology, 337. https://doi.org/10.1186/1746-1596-3-37
Drews, Steven J, et al. "The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens." Diagnostic pathology vol. 3 (2008): 37. doi: https://doi.org/10.1186/1746-1596-3-37
Drews SJ, Eshaghi A, Pyskir D, Chedore P, Lombos E, Broukhanski G, Higgins R, Fisman DN, Blair J, Jamieson F. The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens. Diagn Pathol. 2008 Sep 08;3:37. doi: 10.1186/1746-1596-3-37. PMID: 18778465; PMCID: PMC2546368.
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Diagn Pathol. 2008 Sep 08;3:37. doi: 10.1186/1746-1596-3-37.

The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens.

Diagnostic pathology

Steven J Drews, AliReza Eshaghi, Daria Pyskir, Pam Chedore, Ernesto Lombos, George Broukhanski, Rachel Higgins, David N Fisman, Joanne Blair, Frances Jamieson

Affiliations

  1. Public Health Laboratories Branch, Ministry of Health and Long-Term Care, Toronto, Ontario, Canada. [email protected]

PMID: 18778465 PMCID: PMC2546368 DOI: 10.1186/1746-1596-3-37

Abstract

The Seeplex TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.

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