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Card GP, Vezza AC, Bishop DH, et al. Structural proteins of Tacaribe and Tamiami virions. Virology. 1977;83(1):84-95doi: 10.1016/0042-6822(77)90212-4.
Card, G. P., Vezza, A. C., Bishop, D. H., & Compans, R. W. (1977). Structural proteins of Tacaribe and Tamiami virions. Virology, 83(1), 84-95. https://doi.org/10.1016/0042-6822(77)90212-4
Card, G P, et al. "Structural proteins of Tacaribe and Tamiami virions." Virology vol. 83,1 (1977): 84-95. doi: https://doi.org/10.1016/0042-6822(77)90212-4
Card GP, Vezza AC, Bishop DH, Compans RW. Structural proteins of Tacaribe and Tamiami virions. Virology. 1977 Nov;83(1):84-95. doi: 10.1016/0042-6822(77)90212-4. PMID: 18625477.
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Virology. 1977 Nov;83(1):84-95. doi: 10.1016/0042-6822(77)90212-4.

Structural proteins of Tacaribe and Tamiami virions.

Virology

G P Card, A C Vezza, D H Bishop, R W Compans

Affiliations

  1. Department of Microbiology, The Medical Center, University of Alabama in Birmingham, Birmingham, Alabama 35294, USA.

PMID: 18625477 DOI: 10.1016/0042-6822(77)90212-4

Abstract

Tacaribe and Tamiami viruses were grown in BHK-21 monolayers and purified by centrifugation in combination glycerol-tartrate gradients followed by sucrose gradient centrifugation. The major polypeptides of Tacaribe and Tamiami virions were shown to differ from those of Pichinde virions when suitably labeled preparations of the three viruses were coelectrophoresed in SDS-polyacrylamide gels. Tacaribe and Tamiami virions each contained two major polypeptides, a nonglycosylated protein of molecular weight 68,000 and 66,000, respectively, and a single glycoprotein size-class of molecular weight 42,000 and 44,000, respectively. The nonglycosylated protein is associated with the viral RNA in a nucleoprotein structure which can be isolated by equilibrium centrifugation in CsCl. The glycoproteins were selectively removed from virions by chymotrypsin treatment, which produces essentially spikeless particles. Tacaribe and Tamiami virions also contain a minor polypeptide species of molecular weight 79,000 and 77,000, respectively.

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