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Tsipouri V, Curtin JA, Nolan PM, et al. Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse. Comp Funct Genomics. 2004;5(2):123-7doi: 10.1002/cfg.382.
Tsipouri, V., Curtin, J. A., Nolan, P. M., Vizor, L., Parsons, C. A., Clapham, C. M., Latham, I. D., Rooke, L. J., Martin, J. E., Peters, J., Hunter, A. J., Rogers, D., Rastan, S., Brown, S. D., Fisher, E. M., Spurr, N. K., & Gray, I. C. (2004). Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse. Comparative and functional genomics, 5(2), 123-7. https://doi.org/10.1002/cfg.382
Tsipouri, Vicky, et al. "Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse." Comparative and functional genomics vol. 5,2 (2004): 123-7. doi: https://doi.org/10.1002/cfg.382
Tsipouri V, Curtin JA, Nolan PM, Vizor L, Parsons CA, Clapham CM, Latham ID, Rooke LJ, Martin JE, Peters J, Hunter AJ, Rogers D, Rastan S, Brown SD, Fisher EM, Spurr NK, Gray IC. Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse. Comp Funct Genomics. 2004;5(2):123-7. doi: 10.1002/cfg.382. PMID: 18629060; PMCID: PMC2447344.
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Comp Funct Genomics. 2004;5(2):123-7. doi: 10.1002/cfg.382.

Three novel pigmentation mutants generated by genome-wide random ENU mutagenesis in the mouse.

Comparative and functional genomics

Vicky Tsipouri, John A Curtin, Pat M Nolan, Lucie Vizor, Claire A Parsons, Colin M Clapham, Ian D Latham, Lesley J Rooke, Jo E Martin, Jo Peters, A Jackie Hunter, Derek Rogers, Sohaila Rastan, Steve D M Brown, Elizabeth M C Fisher, Nigel K Spurr, Ian C Gray

Affiliations

  1. GlaxoSmithKline Pharmaceuticals, New Frontiers Science Park, Harlow CM19 5AW, UK. [email protected]

PMID: 18629060 PMCID: PMC2447344 DOI: 10.1002/cfg.382

Abstract

Three mutant mice with pigmentation phenotypes were recovered from a genomewide random mouse chemical mutagenesis study. White toes (Whto; MGI:1861986), Belly spot and white toes (Bswt; MGI:2152776) and Dark footpads 2 (Dfp2; MGI:1861991) were identified following visual inspection of progeny from a male exposed to the point mutagen ethylnitrosourea (ENU). In order to rapidly localize the causative mutations, genome-wide linkage scans were performed on pooled DNA samples from backcross animals for each mutant line. Whto was mapped to proximal mouse chromosome (Mmu) 7 between Cen (the centromere) and D7Mit112 (8.0 cM from the centromere), Bswt was mapped to centric Mmul between D1Mit214 (32.1 cM) and D1Mit480 (32.8 cM) and Dfp2 was mapped to proximalMmu4 between Cen and D4Mit18 (5.2 cM). Whto, Bswt and Dfp2 may provide novel starting points in furthering the elucidation of genetic and biochemical pathways relevant to pigmentation and associated biological processes.

References

  1. Anat Embryol (Berl). 1989;180(5):457-64 - PubMed
  2. Hum Mol Genet. 2000 Jul 22;9(12):1865-71 - PubMed
  3. Genes Dev. 2003 Jan 15;17(2):214-28 - PubMed
  4. Nat Genet. 2000 Aug;25(4):440-3 - PubMed
  5. Annu Rev Genomics Hum Genet. 2001;2:463-92 - PubMed
  6. Nature. 2001 Oct 25;413(6858):856-60 - PubMed
  7. Trends Neurosci. 2003 Jan;26(1):40-5 - PubMed
  8. Cell. 1991 Nov 15;67(4):767-74 - PubMed
  9. J Dermatol Sci. 2002 Jan;28(1):1-33 - PubMed
  10. Semin Cell Dev Biol. 2003 Feb;14(1):19-24 - PubMed
  11. J Inherit Metab Dis. 1998 Aug;21(5):532-9 - PubMed
  12. Curr Opin Genet Dev. 2001 Jun;11(3):268-73 - PubMed
  13. Am J Med Genet. 2000 Winter;97(4):280-8 - PubMed
  14. Genet Res. 1981 Feb;37(1):95-103 - PubMed
  15. Mamm Genome. 1995 Aug;6(8):546-8 - PubMed
  16. Dev Biol. 1997 May 15;185(2):139-47 - PubMed

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