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van der Linden AM, Plasterk RH. Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans. Comp Funct Genomics. 2004;5(3):225-9doi: 10.1002/cfg.392.
van der Linden, A. M., & Plasterk, R. H. (2004). Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans. Comparative and functional genomics, 5(3), 225-9. https://doi.org/10.1002/cfg.392
van der Linden, Alexander M, and Plasterk, Ronald H A. "Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans." Comparative and functional genomics vol. 5,3 (2004): 225-9. doi: https://doi.org/10.1002/cfg.392
van der Linden AM, Plasterk RH. Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans. Comp Funct Genomics. 2004;5(3):225-9. doi: 10.1002/cfg.392. PMID: 18629154; PMCID: PMC2447444.
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Comp Funct Genomics. 2004;5(3):225-9. doi: 10.1002/cfg.392.

Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans.

Comparative and functional genomics

Alexander M van der Linden, Ronald H A Plasterk

Affiliations

  1. Hubrecht Laboratory, Uppsalalaan 8, Utrecht 3584 CT, The Netherlands.

PMID: 18629154 PMCID: PMC2447444 DOI: 10.1002/cfg.392

Abstract

We present a strategy to identify and map large numbers of transposon insertions in the genome of Caenorhabditis elegans. Our approach makes use of the mutator strain mut-7, which has germline-transposition activity of the Tc1/mariner family of transposons, a display protocol to detect new transposon insertions, and the availability of the genomic sequence of C. elegans. From a pilot insertional mutagenesis screen, we have obtained 351 new Tc1 transposons inserted in or near 219 predicted C. elegans genes. The strategy presented provides an approach to isolate insertions of natural transposable elements in many C. elegans genes and to create a large-scale collection of C. elegans mutants.

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