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Stephan CH, Fournier M, Brousseau P, et al. Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum. Chem Cent J. 2008;2:14doi: 10.1186/1752-153X-2-14.
Stephan, C. H., Fournier, M., Brousseau, P., & Sauvé, S. (2008). Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum. Chemistry Central journal, 214. https://doi.org/10.1186/1752-153X-2-14
Stephan, Chadi H, et al. "Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum." Chemistry Central journal vol. 2 (2008): 14. doi: https://doi.org/10.1186/1752-153X-2-14
Stephan CH, Fournier M, Brousseau P, Sauvé S. Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum. Chem Cent J. 2008 Jul 02;2:14. doi: 10.1186/1752-153X-2-14. PMID: 18597692; PMCID: PMC2459166.
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Chem Cent J. 2008 Jul 02;2:14. doi: 10.1186/1752-153X-2-14.

Graphite furnace atomic absorption spectrometry as a routine method for the quantification of beryllium in blood and serum.

Chemistry Central journal

Chadi H Stephan, Michel Fournier, Pauline Brousseau, Sébastien Sauvé

Affiliations

  1. Department of Chemistry, Université de Montréal, Montréal, QC, Canada. [email protected]

PMID: 18597692 PMCID: PMC2459166 DOI: 10.1186/1752-153X-2-14

Abstract

BACKGROUND: A routine method for the quantification of beryllium in biological fluids is essential for the development of a chelation therapy for Chronic Beryllium Disease (CBD). We describe a procedure for the direct determination of beryllium in undigested micro quantities of human blood and serum using graphite furnace atomic absorption spectrometry. Blood and serum samples are prepared respectively by a simple 8-fold and 5-fold dilution with a Nash Reagent. Three experimental setups are compared: using no modifier, using magnesium nitrate and using palladium/citric acid as chemical modifiers.

RESULTS: In serum, both modifiers did not improve the method sensitivity, the optimal pyrolysis and atomization temperatures are 1000 degrees C and 2900 degrees C, respectively. In blood, 6 microg of magnesium nitrate was found to improve the method sensitivity. The optimal pyrolysis and atomization temperatures were 800 degrees C and 2800 degrees C respectively.

CONCLUSION: In serum, the method detection limit was 2 ng l(-1), the characteristic mass was 0.22 (+/- 0.07) pg and the accuracy ranged from 95 to 100%. In blood, the detection limit was 7 ng l(-1), the characteristic mass was 0.20 (+/- 0.02) pg and the accuracy ranged from 99 to 101%.

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