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van der Pol L, Bakker WA, Tramper J. Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnol Bioeng. 1992;40(1):179-82doi: 10.1002/bit.260400125.
van der Pol, L., Bakker, W. A., & Tramper, J. (1992). Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnology and bioengineering, 40(1), 179-82. https://doi.org/10.1002/bit.260400125
van der Pol, L, et al. "Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells." Biotechnology and bioengineering vol. 40,1 (1992): 179-82. doi: https://doi.org/10.1002/bit.260400125
van der Pol L, Bakker WA, Tramper J. Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells. Biotechnol Bioeng. 1992 Jun 05;40(1):179-82. doi: 10.1002/bit.260400125. PMID: 18601060.
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Biotechnol Bioeng. 1992 Jun 05;40(1):179-82. doi: 10.1002/bit.260400125.

Effect of low serum concentrations (0%-2.5%) on growth, production, and shear sensitivity of hybridoma cells.

Biotechnology and bioengineering

L van der Pol, W A Bakker, J Tramper

Affiliations

  1. Bio-Intermediair, Turftorenstr. 12, 9712 BP Groningen, The Netherlands.

PMID: 18601060 DOI: 10.1002/bit.260400125

Abstract

In a stirred culture of hybridoma cells, the effects of serum reduction from 2.5% to 0% on growth and monoclonal antibody production have been investigated. The shear sensitivity of cells from the same culture has been tested in a bubble column. Serum reduction does not greatly affect viable-cell concentrations, but cell specific monoclonal-antibody production rate shows a decreasing trend. A gradual increase in sensitivity for sparging, which is nor the result of a long-term biological effect, has been measured in a bubble column at decreasing fetal calf serum concentrations. Finally, the hypothetical killing-volume model describing the death rate of insect cells in bubble columns has now been completely validated for the pertinent hybridoma-cell line.

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