Display options
Cite
Bloemkolk JW, Gray MR, Merchant F, et al. Effect of temperature on hybridoma cell cycle and MAb production. Biotechnol Bioeng. 1992;40(3):427-31doi: 10.1002/bit.260400312.
Bloemkolk, J. W., Gray, M. R., Merchant, F., & Mosmann, T. R. (1992). Effect of temperature on hybridoma cell cycle and MAb production. Biotechnology and bioengineering, 40(3), 427-31. https://doi.org/10.1002/bit.260400312
Bloemkolk, J W, et al. "Effect of temperature on hybridoma cell cycle and MAb production." Biotechnology and bioengineering vol. 40,3 (1992): 427-31. doi: https://doi.org/10.1002/bit.260400312
Bloemkolk JW, Gray MR, Merchant F, Mosmann TR. Effect of temperature on hybridoma cell cycle and MAb production. Biotechnol Bioeng. 1992 Jul;40(3):427-31. doi: 10.1002/bit.260400312. PMID: 18601133.
Copy Download .nbib Format: NLM
Share it on

Biotechnol Bioeng. 1992 Jul;40(3):427-31. doi: 10.1002/bit.260400312.

Effect of temperature on hybridoma cell cycle and MAb production.

Biotechnology and bioengineering

J W Bloemkolk, M R Gray, F Merchant, T R Mosmann

Affiliations

  1. Department of Chemical Engineering, University of Alberta, Edmonton, Alberta, Canada.

PMID: 18601133 DOI: 10.1002/bit.260400312

Abstract

The kinetics of growth and antibody formation of an anti-interleukin-2 producing hybridoma line were studied in suspension culture at temperatures ranging from 34 degrees C to 39 degrees C. Flow cytometry was used to determine the effect of temperature on the cell cycle. Maximum cell density and monoclonal antibody yield were observed at 37 degrees C. The specific monoclonal antibody production rate was approximately constant throughout each batch experiment. Lower temperatures caused cells to stay longer in the G(1)-phase of the cell cycle, but temperature had only a marginal effect on the specific antibody production rate. Arresting of cells in the G(1)-phase by means of temperature was, therefore, not suited for enhanced monoclonal antibody production. Rather, antibody production for this hybridoma was directly linked to viable cell concentration.

(c) 1992 John Wiley & Sons, Inc.

Publication Types