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Kricka LJ, Marcinkowski JM, Wilding P, et al. Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase. Talanta. 1990;37(10):971-4doi: 10.1016/0039-9140(90)80136-4.
Kricka, L. J., Marcinkowski, J. M., Wilding, P., & Lekhakula, S. (1990). Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase. Talanta, 37(10), 971-4. https://doi.org/10.1016/0039-9140(90)80136-4
Kricka, L J, et al. "Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase." Talanta vol. 37,10 (1990): 971-4. doi: https://doi.org/10.1016/0039-9140(90)80136-4
Kricka LJ, Marcinkowski JM, Wilding P, Lekhakula S. Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase. Talanta. 1990 Oct;37(10):971-4. doi: 10.1016/0039-9140(90)80136-4. PMID: 18965051.
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Talanta. 1990 Oct;37(10):971-4. doi: 10.1016/0039-9140(90)80136-4.

Enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase.

Talanta

L J Kricka, J M Marcinkowski, P Wilding, S Lekhakula

Affiliations

  1. Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA 19104, U.S.A.

PMID: 18965051 DOI: 10.1016/0039-9140(90)80136-4

Abstract

An enhanced chemiluminescence method for determining soluble and immobilized alpha-amylase has been developed, based on use of an insoluble amylose substrate labelled with horseradish peroxidase. Soluble peroxidase-labelled fragments of the substrate, released by the action of alpha-amylase, are quantified by the peroxidase-catalyzed luminol-peroxide-p-iodophenol reaction. The detection limit for alpha-amylase was 125 fmole (12.5 nM). An insoluble amylopectin labelled with horseradish peroxidase was also effective as a substrate for this type of assay.

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