Display options
Cite
Sabry SM, Khamis EF. Application of H-point standard additions method to spectrophotometric and spectrofluorimetric determinations of glafenine and glafenic acid in mixtures. Talanta. 2000;51(6):1219-31doi: 10.1016/s0039-9140(00)00310-6.
Sabry, S. M., & Khamis, E. F. (2000). Application of H-point standard additions method to spectrophotometric and spectrofluorimetric determinations of glafenine and glafenic acid in mixtures. Talanta, 51(6), 1219-31. https://doi.org/10.1016/s0039-9140(00)00310-6
Sabry, S M, and Khamis, E F. "Application of H-point standard additions method to spectrophotometric and spectrofluorimetric determinations of glafenine and glafenic acid in mixtures." Talanta vol. 51,6 (2000): 1219-31. doi: https://doi.org/10.1016/s0039-9140(00)00310-6
Sabry SM, Khamis EF. Application of H-point standard additions method to spectrophotometric and spectrofluorimetric determinations of glafenine and glafenic acid in mixtures. Talanta. 2000 May 05;51(6):1219-31. doi: 10.1016/s0039-9140(00)00310-6. PMID: 18967954.
Copy Download .nbib Format: NLM
Share it on

Talanta. 2000 May 05;51(6):1219-31. doi: 10.1016/s0039-9140(00)00310-6.

Application of H-point standard additions method to spectrophotometric and spectrofluorimetric determinations of glafenine and glafenic acid in mixtures.

Talanta

S M Sabry, E F Khamis

Affiliations

  1. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.

PMID: 18967954 DOI: 10.1016/s0039-9140(00)00310-6

Abstract

H-point standard additions method (HPSAM), based on spectrophotometric and spectrofluorimetric measurements, was proposed for simultaneous determination of glafenine (G) and glafenic acid (GA). A study of the absorption spectra of G and GA in various pH media has been carried out. Reasonably resolved UV-absorption spectra were obtained with a solution adjusted at pH 4.5 with citric acid-phosphate buffer. Additionally, the fluorescence properties in aqueous micellar systems of anionic, cationic and non-ionic surfactants were investigated. Well resolved fluorescence spectra were established in aqueous Triton X-100 solution at pH 7.8 (citric acid-phosphate buffer). As a comparative method, UV-derivative spectrophotometry (based on zero-crossing technique) was suggested. First-derivative value at 352 nm ((1)D(352)) and second-derivative value at 366 nm ((2)D(366)) were selected for the quantification of G and GA, respectively. The relative standard deviations of the proposed methods approximate 2%. The proposed methods were evaluated through the analysis of commercial tablets. The results were accurate and precise.

Publication Types