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Sakamoto A, Murata K, Suzuki H, et al. Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis. Acta Histochem Cytochem. 2008;41(5):143-7doi: 10.1267/ahc.08026.
Sakamoto, A., Murata, K., Suzuki, H., Yatabe, M., & Kikuchi, M. (2008). Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis. Acta histochemica et cytochemica, 41(5), 143-7. https://doi.org/10.1267/ahc.08026
Sakamoto, Atsushi, et al. "Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis." Acta histochemica et cytochemica vol. 41,5 (2008): 143-7. doi: https://doi.org/10.1267/ahc.08026
Sakamoto A, Murata K, Suzuki H, Yatabe M, Kikuchi M. Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis. Acta Histochem Cytochem. 2008 Oct 29;41(5):143-7. doi: 10.1267/ahc.08026. Epub 2008 Oct 10. PMID: 18989468; PMCID: PMC2576505.
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Acta Histochem Cytochem. 2008 Oct 29;41(5):143-7. doi: 10.1267/ahc.08026. Epub 2008 Oct 10.

Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis.

Acta histochemica et cytochemica

Atsushi Sakamoto, Kazumoto Murata, Hideto Suzuki, Megumi Yatabe, Motoshi Kikuchi

Affiliations

  1. Division of Forensic Medicine, Center for Community Medicine, Jichi Medical University School of Medicine, Tochigi, Japan. [email protected]

PMID: 18989468 PMCID: PMC2576505 DOI: 10.1267/ahc.08026

Abstract

Cadherins are a family of transmembrane glycoproteins that mediate cell-to-cell adhesion. Isoforms, including E- and N-cadherin, have been identified and shown to regulate morphogenesis through homophilic binding. In the ontogeny, the expressions of E- and N-cadherin change spatiotemporally, and the changes in cadherin isoforms, called cadherin switching, impact the mechanical adhesion of cells. Furthermore, cadherin functions as a receptor that transfers information from outside to inside cells, and in terms of switching, it affects cell phenotypes. To observe the expression patterns of E- and N-cadherins during embryogenesis and to identify cells that transiently coexpress both cadherins, we employed a recently developed immunohistochemical double staining technique in rat fetuses. At embryonic day 9, embryonic ectodermal cells more dominantly expressed E-cadherin, while mesodermal cells more dominantly expressed N-cadherin. At embryonic day 10, the expression pattern of E-cadherin in the surface ectoderm and endoderm and that of N-cadherin in the neuroectoderm were established. After embryonic day 10, unique co-expression of E- and N-cadherin was observed in primordia, such as the bulbus cordis, otic pit, notochord, and Rathke's pouch. In the present study, it was possible to visualize the expression patterns of E- and N-cadherin during early fetal development, which enabled us to morphologically clarify cadherin switching.

Keywords: cadherin switching; immunohistochemistry; organogenesis; rat

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