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Rodriguez-Mozaz S, de Alda MJ, Barceló D. Fast and simultaneous monitoring of organic pollutants in a drinking water treatment plant by a multi-analyte biosensor followed by LC-MS validation. Talanta. 2005;69(2):377-84doi: 10.1016/j.talanta.2005.09.050.
Rodriguez-Mozaz, S., de Alda, M. J., & Barceló, D. (2006). Fast and simultaneous monitoring of organic pollutants in a drinking water treatment plant by a multi-analyte biosensor followed by LC-MS validation. Talanta, 69(2), 377-84. https://doi.org/10.1016/j.talanta.2005.09.050
Rodriguez-Mozaz, Sara, et al. "Fast and simultaneous monitoring of organic pollutants in a drinking water treatment plant by a multi-analyte biosensor followed by LC-MS validation." Talanta vol. 69,2 (2006): 377-84. doi: https://doi.org/10.1016/j.talanta.2005.09.050
Rodriguez-Mozaz S, de Alda MJ, Barceló D. Fast and simultaneous monitoring of organic pollutants in a drinking water treatment plant by a multi-analyte biosensor followed by LC-MS validation. Talanta. 2006 Apr 15;69(2):377-84. doi: 10.1016/j.talanta.2005.09.050. Epub 2005 Nov 14. PMID: 18970577.
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Talanta. 2006 Apr 15;69(2):377-84. doi: 10.1016/j.talanta.2005.09.050. Epub 2005 Nov 14.

Fast and simultaneous monitoring of organic pollutants in a drinking water treatment plant by a multi-analyte biosensor followed by LC-MS validation.

Talanta

Sara Rodriguez-Mozaz, Maria J López de Alda, Damià Barceló

Affiliations

  1. Department of Environmental Chemistry, IIQAB-Consejo Superior de Investigaciones Cientificas, C/ Jordi Girona Salgado 18-26, Barcelona, Spain.

PMID: 18970577 DOI: 10.1016/j.talanta.2005.09.050

Abstract

This work describes the application of an optical biosensor (RIver ANALyser, RIANA) to the simultaneous analysis of three relevant environmental organic pollutants, namely, the pesticides atrazine and isoproturon and the estrogen estrone, in real water samples. This biosensor is based on an indirect inhibition immunoassay which takes place at a chemically modified optical transducer chip. The spatially resolved modification of the transducer surface allows the simultaneous determination of selected target analytes by means of "total internal reflection fluorescence" (TIRF). The performance of the immunosensor method developed was evaluated against a well accepted traditional method based on solid-phase extraction followed by liquid chromatography-mass spectrometry (LC-MS). The chromatographic method was superior in terms of linearity, sensitivity and accuracy, and the biosensor method in terms of repeatability, speed, cost and automation. The application of both methods in parallel to determine the occurrence and removal of atrazine, isoproturon and estrone throughout the treatment process (sand filtration, ozonation, activated carbon filtration and chlorination) in a waterworks showed an overestimation of results in the case of the biosensor, which was partially attributed to matrix and cross-reactivity effects, in spite of the addition of ovalbumin to the sample to minimize matrix interferences. Based on the comparative performance of both techniques, the biosensor emerges as a suitable tool for fast, simple and automated screening of water pollutants without sample pretreatment. To the author's knowledge, this is the first description of the application of the biosensor RIANA in the multi-analyte configuration to the regular monitoring of pollutants in a waterworks.

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