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Huang Y, Jiang X, Wang W, et al. Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection. Talanta. 2006;70(5):1157-63doi: 10.1016/j.talanta.2006.03.009.
Huang, Y., Jiang, X., Wang, W., Duan, J., & Chen, G. (2006). Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection. Talanta, 70(5), 1157-63. https://doi.org/10.1016/j.talanta.2006.03.009
Huang, Ying, et al. "Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection." Talanta vol. 70,5 (2006): 1157-63. doi: https://doi.org/10.1016/j.talanta.2006.03.009
Huang Y, Jiang X, Wang W, Duan J, Chen G. Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection. Talanta. 2006 Dec 15;70(5):1157-63. doi: 10.1016/j.talanta.2006.03.009. Epub 2006 Jun 14. PMID: 18970894.
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Talanta. 2006 Dec 15;70(5):1157-63. doi: 10.1016/j.talanta.2006.03.009. Epub 2006 Jun 14.

Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection.

Talanta

Ying Huang, Xiuyan Jiang, Wei Wang, Jianping Duan, Guonan Chen

Affiliations

  1. The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China; College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian 350007, China.

PMID: 18970894 DOI: 10.1016/j.talanta.2006.03.009

Abstract

A method of capillary electrophoresis with wall-jet amperometric detection (AD) has been developed for separation and determination of l-tyrosine (Tyr) and its metabolites, such as Tyramine (TA), p-hydroxyphenylpyruvic (pHPP), homogentisic acid (HGA) and some dipeptides containing Tyr, such as Tyr-Gly-Gly (YGG), Tyr-Arg (YR) and Tyr-d-Arg (Y-d-R). A carbon disk electrode was used as the working electrode and the optimal detection potential was 1.00V (versus Ag/AgCl). At 18kV of applied voltage, the seven compounds were completely separated within 20min in 110x10(-3)mol/L Na(2)HPO(4)-NaH(2)PO(4) buffer (pH 7.10) containing 3x10(-3)mol/L beta-cyclodextrin (beta-CD). Good linear relationship was obtained for all analytes and the detection limits of seven analytes were in the range of 0.95-4.25ng/mL. The proposed method has been applied to examine the metabolic process of l-tyrosine in rabbit's urine.

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