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Luo CX, Yin LF, Koyanagi S, et al. Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice. Phytopathology. 2005;95(6):640-7doi: 10.1094/PHYTO-95-0640.
Luo, C. X., Yin, L. F., Koyanagi, S., Farman, M. L., Kusaba, M., & Yaegashi, H. (2005). Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice. Phytopathology, 95(6), 640-7. https://doi.org/10.1094/PHYTO-95-0640
Luo, Chao-Xi, et al. "Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice." Phytopathology vol. 95,6 (2005): 640-7. doi: https://doi.org/10.1094/PHYTO-95-0640
Luo CX, Yin LF, Koyanagi S, Farman ML, Kusaba M, Yaegashi H. Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice. Phytopathology. 2005 Jun;95(6):640-7. doi: 10.1094/PHYTO-95-0640. PMID: 18943780.
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Phytopathology. 2005 Jun;95(6):640-7. doi: 10.1094/PHYTO-95-0640.

Genetic Mapping and Chromosomal Assignment of Magnaporthe oryzae Avirulence Genes AvrPik, AvrPiz, and AvrPiz-t Controlling Cultivar Specificity on Rice.

Phytopathology

Chao-Xi Luo, Liang-Fen Yin, Satomi Koyanagi, Mark L Farman, Motoaki Kusaba, Hiroshi Yaegashi

PMID: 18943780 DOI: 10.1094/PHYTO-95-0640

Abstract

ABSTRACT A genetic map including three avirulence (Avr) genes, AvrPik, AvrPiz, and AvrPiz-t, was constructed in a genetic cross of two rice field isolates, 84R-62B and Y93-245c-2. The chromosomal locations of the Avr genes were determined by using selected markers to probe Southern blots of the parental chromosomes that had been separated by contour-clamped homogenous electric fields electrophoresis. Electrophoretic karyotyping showed that both parental isolates 84R-62B and Y93-245c-2 contained seven chromosomes greater than 3.5 megabases (Mb) in size and 84R-62B possessed a small chromosome of approximately 1.6 Mb. The linkage groups containing AvrPiz and AvrPiz-t were assigned to chromosomes 3 and 7, respectively. Some markers from the linkage group that contained AvrPik hybridized with chromosome 1 and the 1.6-Mb chromosome, yet all of the cloned RAPD markers that were closely linked to AvrPik hybridized exclusively to the 1.6-Mb chromosome in 84R-62B, the parent that possesses AvrPik. Thus, we conclude that AvrPik is located on the 1.6-Mb chromosome in 84R-62B.

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