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Dutra PM, Dias FA, Santos MA, et al. Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor. Phytopathology. 2001;91(4):408-14doi: 10.1094/PHYTO.2001.91.4.408.
Dutra, P. M., Dias, F. A., Santos, M. A., Rodrigues, C. O., Romeiro, A., Attias, M., De Souza, W., Lopes, A. H., & Meyer-Fernandes, J. R. (2001). Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor. Phytopathology, 91(4), 408-14. https://doi.org/10.1094/PHYTO.2001.91.4.408
Dutra, P M, et al. "Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor." Phytopathology vol. 91,4 (2001): 408-14. doi: https://doi.org/10.1094/PHYTO.2001.91.4.408
Dutra PM, Dias FA, Santos MA, Rodrigues CO, Romeiro A, Attias M, De Souza W, Lopes AH, Meyer-Fernandes JR. Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor. Phytopathology. 2001 Apr;91(4):408-14. doi: 10.1094/PHYTO.2001.91.4.408. PMID: 18943854.
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Phytopathology. 2001 Apr;91(4):408-14. doi: 10.1094/PHYTO.2001.91.4.408.

Secreted phosphatase activities in trypanosomatid parasites of plants modulated by platelet-activating factor.

Phytopathology

P M Dutra, F A Dias, M A Santos, C O Rodrigues, A Romeiro, M Attias, W De Souza, A H Lopes, J R Meyer-Fernandes

PMID: 18943854 DOI: 10.1094/PHYTO.2001.91.4.408

Abstract

ABSTRACT The secreted phosphatase activities of two trypanosomatid parasites were characterized and compared with supernatants of living cells. The plant parasite Phytomonas françai and the phytophagous hemipteran parasite Herpetomonas sp. hydrolyzed p-nitrophenylphosphate at a rate of 15.54 and 6.51 nmol Pi/mg of protein per min, respectively. Sodium orthovanadate (N(a)VO(3)) and sodium fluoride (NaF) decreased the phosphatase activities. The phosphatase activity of P. françai was drastically diminished (73% inhibition) in the presence of sodium tartrate, whereas the phosphatase activity of Herpetomonas sp. was inhibited by 23%. Cytochemical analysis showed the localization of these enzymes on the external surface and in the flagellar pocket of the two trypanosomatids. Sodium tartrate inhibited this reaction, confirming the biochemical data. Platelet-activating factor modulated the phosphatase activities, inhibiting P. françai activity and stimulating Herpetomonas sp. phosphatase activity.

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