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Chen ZY, Brown RL, Rajasekaran K, et al. Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production. Phytopathology. 2006;96(1):87-95doi: 10.1094/PHYTO-96-0087.
Chen, Z. Y., Brown, R. L., Rajasekaran, K., Damann, K. E., & Cleveland, T. E. (2006). Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production. Phytopathology, 96(1), 87-95. https://doi.org/10.1094/PHYTO-96-0087
Chen, Z-Y, et al. "Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production." Phytopathology vol. 96,1 (2006): 87-95. doi: https://doi.org/10.1094/PHYTO-96-0087
Chen ZY, Brown RL, Rajasekaran K, Damann KE, Cleveland TE. Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production. Phytopathology. 2006 Jan;96(1):87-95. doi: 10.1094/PHYTO-96-0087. PMID: 18944208.
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Phytopathology. 2006 Jan;96(1):87-95. doi: 10.1094/PHYTO-96-0087.

Identification of a Maize Kernel Pathogenesis-Related Protein and Evidence for Its Involvement in Resistance to Aspergillus flavus Infection and Aflatoxin Production.

Phytopathology

Z-Y Chen, R L Brown, K Rajasekaran, K E Damann, T E Cleveland

Affiliations

  1. First and fourth authors: Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803 ; and second, third, and fifth authors: Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, New Orleans, LA 70179.

PMID: 18944208 DOI: 10.1094/PHYTO-96-0087

Abstract

ABSTRACT Aflatoxins are carcinogens produced by Aspergillus flavus and A. parasiticus during infection of susceptible crops such as maize. Several aflatoxin-resistant maize genotypes have been identified and kernel proteins have been suggested to play an important role in resistance. In the present study, one protein (#717), which was expressed fivefold higher in three resistant lines compared with three susceptible ones, was identified using proteomics. This protein was sequenced and identified as a pathogenesis-related protein (PR-10) based on its sequence homology. To assess the involvement of this PR-10 protein (ZmPR-10) in host resistance of maize against fungal infection and aflatoxin production, the corresponding cDNA (pr-10) was cloned. It encodes a protein of 160 amino acids with a predicted molecular mass of 16.9 kDa and an iso-electric point of 5.38. The expression of pr-10 during kernel development increased fivefold between 7 and 22 days after pollination, and was induced upon A. flavus infection in the resistant but not in the susceptible genotype. The ZmPR-10 overexpressed in Escherichia coli exhibited a ribonucleolytic and antifungal activities. Leaf extracts of transgenic tobacco plants expressing maize pr-10 also demonstrated RNase activity and inhibited the growth of A. flavus. This evidence suggests that ZmPR-10 plays a role in kernel resistance by inhibiting fungal growth of A. flavus.

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