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Louws FJ, Bell J, Medina-Mora CM, et al. rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis. Phytopathology. 1998;88(8):862-8doi: 10.1094/PHYTO.1998.88.8.862.
Louws, F. J., Bell, J., Medina-Mora, C. M., Smart, C. D., Opgenorth, D., Ishimaru, C. A., Hausbeck, M. K., de Bruijn, F. J., & Fulbright, D. W. (1998). rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis. Phytopathology, 88(8), 862-8. https://doi.org/10.1094/PHYTO.1998.88.8.862
Louws, F J, et al. "rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis." Phytopathology vol. 88,8 (1998): 862-8. doi: https://doi.org/10.1094/PHYTO.1998.88.8.862
Louws FJ, Bell J, Medina-Mora CM, Smart CD, Opgenorth D, Ishimaru CA, Hausbeck MK, de Bruijn FJ, Fulbright DW. rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis. Phytopathology. 1998 Aug;88(8):862-8. doi: 10.1094/PHYTO.1998.88.8.862. PMID: 18944895.
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Phytopathology. 1998 Aug;88(8):862-8. doi: 10.1094/PHYTO.1998.88.8.862.

rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis.

Phytopathology

F J Louws, J Bell, C M Medina-Mora, C D Smart, D Opgenorth, C A Ishimaru, M K Hausbeck, F J de Bruijn, D W Fulbright

PMID: 18944895 DOI: 10.1094/PHYTO.1998.88.8.862

Abstract

ABSTRACT The genomic DNA fingerprinting technique known as repetitive-sequence-based polymerase chain reaction (rep-PCR) was evaluated as a tool to differentiate subspecies of Clavibacter michiganensis, with special emphasis on C. michiganensis subsp. michiganensis, the pathogen responsible for bacterial canker of tomato. DNA primers (REP, ERIC, and BOX), corresponding to conserved repetitive element motifs in the genomes of diverse bacterial species, were used to generate genomic fingerprints of C. michiganensis subsp. michiganensis, C. michiganensis subsp. sepedonicus, C. michiganensis subsp. nebraskensis, C. michiganensis subsp. tessellarius, and C. michiganensis subsp. insidiosum. The rep-PCR-generated patterns of DNA fragments observed after agarose gel electrophoresis support the current division of C. michiganensis into five subspecies. In addition, the rep-PCR fingerprints identified at least four types (A, B, C, and D) within C. michiganensis subsp. michiganensis based on limited DNA polymorphisms; the ability to differentiate individual strains may be of potential use in studies on the epidemiology and host-pathogen interactions of this organism. In addition, we have recovered from diseased tomato plants a relatively large number of naturally occurring avirulent C. michiganensis subsp. michiganensis strains with rep-PCR fingerprints identical to those of virulent C. michiganensis subsp. michiganensis strains.

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