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Neumann H, Bode-Kirchhoff A, Madeheim A, et al. Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds. Environ Sci Pollut Res Int. 1998;5(1):28-36doi: 10.1007/BF02986371.
Neumann, H., Bode-Kirchhoff, A., Madeheim, A., & Wetzel, A. (1998). Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds. Environmental science and pollution research international, 5(1), 28-36. https://doi.org/10.1007/BF02986371
Neumann, H, et al. "Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds." Environmental science and pollution research international vol. 5,1 (1998): 28-36. doi: https://doi.org/10.1007/BF02986371
Neumann H, Bode-Kirchhoff A, Madeheim A, Wetzel A. Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds. Environ Sci Pollut Res Int. 1998;5(1):28-36. doi: 10.1007/BF02986371. PMID: 19002625.
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Environ Sci Pollut Res Int. 1998;5(1):28-36. doi: 10.1007/BF02986371.

Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds.

Environmental science and pollution research international

H Neumann, A Bode-Kirchhoff, A Madeheim, A Wetzel

Affiliations

  1. FG Zellbiologie und Angewandte Botanik, Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-35032, Marburg, Germany.

PMID: 19002625 DOI: 10.1007/BF02986371

Abstract

Legume root nodules are the site of biological nitrogen fixation in the Rhizobium-legume symbiosis. Nodules are structures unique to this symbiosis and they are morphologically as well as physiologically distinct from other plant organs. Organic substances affecting the macro- or microsymbionts vitality, such as PAHs (WETZEL: et al., 1991), reduce nodulation even before visible damage to the plant can be detected. We present data that the formation of nodules (nodulation) may also serve for ecotoxicological evaluation of heavy metals in different binding states. Tests were performed in petri dishes with alfalfa (lucerne) seedlings inoculated with Rhizobium meliloti. Cultivation took place in growth cabinets with carefully standardized and documented growth conditions. Data from stressed plants was recorded after 14 days of cultivation on contaminated substrate. A dose responsive decrease in nodulation was found after application of cadmium acetate, cadmium iodide, cadmium chloride, sodium salts of arsenate and arsenite, arsenic pentoxide, and lead nitrate, whereas lead acetate showed no effect up to a concentration of 3 microM. The dose response curves were used to calculate EC10, EC50 and EC90 values. EC50 values for cadmium compounds range from 1.5 to 9.5 pM. Testing different arsenic compounds results in EC50 from 2.6 to 20.1 microM. EC50 of lead nitrate is 2.2 microM. The sensitivity, reproducibility and reliability of this test system is discussed compared to established biotests.

References

  1. Bull Environ Contam Toxicol. 1995 May;54(5):633-9 - PubMed
  2. Plant Physiol. 1991 Nov;97(3):1221-5 - PubMed
  3. IARC Monogr Eval Carcinog Risk Chem Hum. 1980;23:1-415 - PubMed
  4. Arch Environ Contam Toxicol. 1993 Aug;25(2):250-4 - PubMed
  5. Appl Environ Microbiol. 1991 Aug;57(8):2205-10 - PubMed
  6. Plant Physiol. 1988 Oct;88(2):396-400 - PubMed
  7. IARC Monogr Eval Carcinog Risks Hum. 1993;58:1-415 - PubMed

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