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Cunningham SC, Lew AM, Tannock GA. The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). Avian Pathol. 2001;30(6):613-9doi: 10.1080/03079450120092107.
Cunningham, S. C., Lew, A. M., & Tannock, G. A. (2001). The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). Avian pathology : journal of the W.V.P.A, 30(6), 613-9. https://doi.org/10.1080/03079450120092107
Cunningham, S C, et al. "The antigenicity of the chicken anaemia virus protein VP3 (Apoptin)." Avian pathology : journal of the W.V.P.A vol. 30,6 (2001): 613-9. doi: https://doi.org/10.1080/03079450120092107
Cunningham SC, Lew AM, Tannock GA. The antigenicity of the chicken anaemia virus protein VP3 (Apoptin). Avian Pathol. 2001 Dec;30(6):613-9. doi: 10.1080/03079450120092107. PMID: 19184955.
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Avian Pathol. 2001 Dec;30(6):613-9. doi: 10.1080/03079450120092107.

The antigenicity of the chicken anaemia virus protein VP3 (Apoptin).

Avian pathology : journal of the W.V.P.A

S C Cunningham, A M Lew, G A Tannock

PMID: 19184955 DOI: 10.1080/03079450120092107

Abstract

Chicken anaemia virus protein VP3 (Apoptin) was cloned and expressed as a recombinant protein and evaluated for its suitability as a serodiagnostic reagent. VP3 was expressed as a fusion protein either with glutathione S -transferase or with a six-histidine tag. Both recombinant proteins reacted specifically with anti-VP3 monoclonal antibodies and with serum from vaccinated chickens by Western blot and by enzyme-linked immunosorbent assay (ELISA). However, when testing sera from birds of different ages and genetic backgrounds, high non-specific reactions were evident and false positives were observed, especially in older birds. This suggests that VP3 is poorly immunogenic during infection and low antibody concentrations are masked by non-specific reactions. Thus, VP3 is not suitable for use as antigen in ELISAs.

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