Display options
Cite
Almeda S, Arce L, Benavente F, et al. Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound. Anal Bioanal Chem. 2009;394(2):609-15doi: 10.1007/s00216-009-2696-x.
Almeda, S., Arce, L., Benavente, F., Sanz-Nebot, V., Barbosa, J., & Valcárcel, M. (2009). Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound. Analytical and bioanalytical chemistry, 394(2), 609-15. https://doi.org/10.1007/s00216-009-2696-x
Almeda, S, et al. "Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound." Analytical and bioanalytical chemistry vol. 394,2 (2009): 609-15. doi: https://doi.org/10.1007/s00216-009-2696-x
Almeda S, Arce L, Benavente F, Sanz-Nebot V, Barbosa J, Valcárcel M. Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound. Anal Bioanal Chem. 2009 May;394(2):609-15. doi: 10.1007/s00216-009-2696-x. Epub 2009 Feb 28. PMID: 19252901.
Copy Download .nbib Format: NLM
Share it on

Anal Bioanal Chem. 2009 May;394(2):609-15. doi: 10.1007/s00216-009-2696-x. Epub 2009 Feb 28.

Comparison of off- and in-line solid-phase extraction for enhancing sensitivity in capillary electrophoresis using ochratoxin as a model compound.

Analytical and bioanalytical chemistry

S Almeda, L Arce, F Benavente, V Sanz-Nebot, J Barbosa, M Valcárcel

Affiliations

  1. Department of Analytical Chemistry, University of Córdoba, Campus de Rabanales, 14071, Córdoba, Spain.

PMID: 19252901 DOI: 10.1007/s00216-009-2696-x

Abstract

This paper proposes and compares two approaches based on off- and in-line solid-phase extraction (SPE), intended to enhance sensitivity in capillary electrophoresis with ultraviolet detection (CE-UV) using as a model the determination of ochratoxin A (OA) in river water samples. In the off-line SPE mode, the reversed-phase sorbent (octadecilsylane, C(18)) selectively retains the target analyte (OA) and allows large volumes of the sample (70 mL) to be introduced and subsequently eluted in a small volume (0.1 mL) of an appropriate solution. In the in-line SPE mode, a custom-made microcartridge is inserted near the inlet of the capillary, which is filled with the same C(18) sorbent. This solid phase selectively retains OA present in a sample volume as low as approximately 640 microL for subsequent elution with ca. 135 nL of an appropriate eluent. The limit of detection (LOD) obtained with the in-line SPE method was 1 ng L(-1), which is 3 orders of magnitude lower than that obtained with CE-UV and roughly 1 order lower than that provided by the off-line SPE-CE-UV method.

Publication Types