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Habermann N, Lund EK, Pool-Zobel BL, et al. Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells. Genes Nutr. 2009;4(1):73-6doi: 10.1007/s12263-009-0112-y.
Habermann, N., Lund, E. K., Pool-Zobel, B. L., & Glei, M. (2009). Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells. Genes & nutrition, 4(1), 73-6. https://doi.org/10.1007/s12263-009-0112-y
Habermann, Nina, et al. "Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells." Genes & nutrition vol. 4,1 (2009): 73-6. doi: https://doi.org/10.1007/s12263-009-0112-y
Habermann N, Lund EK, Pool-Zobel BL, Glei M. Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells. Genes Nutr. 2009 Mar;4(1):73-6. doi: 10.1007/s12263-009-0112-y. Epub 2009 Feb 21. PMID: 19234733; PMCID: PMC2654050.
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Genes Nutr. 2009 Mar;4(1):73-6. doi: 10.1007/s12263-009-0112-y. Epub 2009 Feb 21.

Modulation of gene expression in eicosapentaenoic acid and docosahexaenoic acid treated human colon adenoma cells.

Genes & nutrition

Nina Habermann, Elizabeth K Lund, Beatrice L Pool-Zobel, Michael Glei

Affiliations

  1. Department of Nutritional Toxicology, Institute for Nutrition, Friedrich-Schiller-University Jena, Dornburger Strasse 24, 07743, Jena, Germany, [email protected].

PMID: 19234733 PMCID: PMC2654050 DOI: 10.1007/s12263-009-0112-y

Abstract

Epidemiological studies suggest that high fish intake is associated with a decreased risk of colorectal cancer which has been linked to the high content of the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acids (EPA) and docosahexaenoic acid (DHA) in some fish. The aim of the study was to compare the modulation of gene expression in LT97 colon adenoma cells in response to EPA and DHA treatment. Therefore, we used custom-designed cDNA arrays containing probes for 306 genes related to stress response, apoptosis and carcinogenesis and hybridised them with cDNA from LT97 cells which were treated for 10 or 24 h with 50 muM EPA or DHA. There was a marked influence of n-3 PUFA on the expression of several gene types, such as detoxification, cell cycle control, signaling pathways, apoptosis and inflammation. DHA and EPA generally modulated different sets of genes, although a few common effects were noted. In our approach, we used preneoplastic adenoma cells which are a relevant model for target cells of chemoprevention. If verified with real time PCR, these results identify genes and targets for chemoprevention of colon cancer.

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