Display options
Cite
Shurubor YI, Matson WR, Willett WC, et al. Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome. BMC Clin Pathol. 2007;7:9doi: 10.1186/1472-6890-7-9.
Shurubor, Y. I., Matson, W. R., Willett, W. C., Hankinson, S. E., & Kristal, B. S. (2007). Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome. BMC clinical pathology, 79. https://doi.org/10.1186/1472-6890-7-9
Shurubor, Yevgeniya I, et al. "Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome." BMC clinical pathology vol. 7 (2007): 9. doi: https://doi.org/10.1186/1472-6890-7-9
Shurubor YI, Matson WR, Willett WC, Hankinson SE, Kristal BS. Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome. BMC Clin Pathol. 2007 Nov 12;7:9. doi: 10.1186/1472-6890-7-9. PMID: 17997839; PMCID: PMC2203971.
Copy Download .nbib Format: NLM
Share it on

BMC Clin Pathol. 2007 Nov 12;7:9. doi: 10.1186/1472-6890-7-9.

Biological variability dominates and influences analytical variance in HPLC-ECD studies of the human plasma metabolome.

BMC clinical pathology

Yevgeniya I Shurubor, Wayne R Matson, Walter C Willett, Susan E Hankinson, Bruce S Kristal

Affiliations

  1. Department of Neurosurgery, Brigham and Women's Hospital, 221 Longwood Ave, LM322B, Boston, MA 02115, USA. [email protected]

PMID: 17997839 PMCID: PMC2203971 DOI: 10.1186/1472-6890-7-9

Abstract

BACKGROUND: Biomarker-based assessments of biological samples are widespread in clinical, pre-clinical, and epidemiological investigations. We previously developed serum metabolomic profiles assessed by HPLC-separations coupled with coulometric array detection that can accurately identify ad libitum fed and caloric-restricted rats. These profiles are being adapted for human epidemiology studies, given the importance of energy balance in human disease.

METHODS: Human plasma samples were biochemically analyzed using HPLC separations coupled with coulometric electrode array detection.

RESULTS: We identified these markers/metabolites in human plasma, and then used them to determine which human samples represent blinded duplicates with 100% accuracy (N = 30 of 30). At least 47 of 61 metabolites tested were sufficiently stable for use even after 48 hours of exposure to shipping conditions. Stability of some metabolites differed between individuals (N = 10 at 0, 24, and 48 hours), suggesting the influence of some biological factors on parameters normally considered as analytical.

CONCLUSION: Overall analytical precision (mean median CV, ~9%) and total between-person variation (median CV, ~50-70%) appear well suited to enable use of metabolomics markers in human clinical trials and epidemiological studies, including studies of the effect of caloric intake and balance on long-term cancer risk.

References

  1. J Nutr. 2003 Mar;133 Suppl 3:895S-920S - PubMed
  2. Int J Epidemiol. 2003 Feb;32(1):125-30 - PubMed
  3. Am J Epidemiol. 2003 Jul 1;158(1):1-13 - PubMed
  4. Biometrics. 2008 Mar;64(1):289-98 - PubMed
  5. Am J Clin Nutr. 2001 Aug;74(2):188-96 - PubMed
  6. OMICS. 2004 Fall;8(3):197-208 - PubMed
  7. Trends Biotechnol. 2004 May;22(5):245-52 - PubMed
  8. Methods Mol Biol. 2002;186:185-94 - PubMed
  9. Nat Biotechnol. 2000 Nov;18(11):1157-61 - PubMed
  10. J Am Diet Assoc. 2006 Oct;106(10):1640-50 - PubMed
  11. Br J Nutr. 2001 Aug;86 Suppl 1:S37-53 - PubMed
  12. Anal Biochem. 1998 Oct 1;263(1):18-25 - PubMed
  13. J Nutr. 2001 Mar;131(3):924S-932S - PubMed
  14. J Nutr. 2002 May;132(5):1031-8 - PubMed
  15. Clin Chem Lab Med. 2004;42(7):858-62 - PubMed
  16. Clin Chim Acta. 2004 Aug 2;346(1):19-24 - PubMed
  17. Int J Epidemiol. 1997;26 Suppl 1:S137-51 - PubMed
  18. Clin Chem. 1989 Dec;35(12):2313-6 - PubMed
  19. J Nutr. 2003 Jun;133(6 Suppl 1):2078S-2083S - PubMed
  20. Toxicol Lett. 1995 May;77(1-3):195-204 - PubMed
  21. Public Health Nutr. 2008 Feb;11(2):183-95 - PubMed
  22. Clin Chem. 2006 Apr;52(4):601-23 - PubMed
  23. Eur J Clin Nutr. 2003 Nov;57(11):1370-7 - PubMed
  24. J Nutr. 2003 Jun;133(6 Suppl 1):2097S-2100S - PubMed
  25. Eur J Clin Nutr. 2008 Nov;62(11):1343-50 - PubMed
  26. J Nutr. 2002 Sep;132(9):2486-7 - PubMed
  27. J Nutr. 2003 Mar;133(3):873S-874S - PubMed
  28. N Engl J Med. 1999 Aug 5;341(6):427-34 - PubMed
  29. Anal Biochem. 2002 Mar 15;302(2):239-45 - PubMed
  30. J Natl Cancer Inst. 2004 Sep 1;96(17):1276-87 - PubMed
  31. Br J Nutr. 2001 Aug;86 Suppl 1:S5-35 - PubMed
  32. J Nutr. 2002 May;132(5):1039-46 - PubMed
  33. Ann Clin Biochem. 2002 Nov;39(Pt 6):543-50 - PubMed
  34. Am J Clin Nutr. 2007 Aug;86(2):382-7 - PubMed
  35. Int J Obes (Lond). 2007 Jun;31(6):956-61 - PubMed
  36. IARC Sci Publ. 1997;(142):303-12 - PubMed
  37. OMICS. 2004 Fall;8(3):209-20 - PubMed
  38. OMICS. 2004 Fall;8(3):221-38 - PubMed
  39. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Oct 15;810(1):93-100 - PubMed
  40. J Nutr. 2003 Mar;133 Suppl 3:888S-894S - PubMed
  41. Plant Mol Biol. 2002 Jan;48(1-2):155-71 - PubMed
  42. Trends Plant Sci. 2004 Sep;9(9):418-25 - PubMed
  43. Gynecol Oncol. 1999 Jul;74(1):12-22 - PubMed
  44. Curr Opin Biotechnol. 2002 Oct;13(5):512-6 - PubMed
  45. Curr Opin Mol Ther. 2002 Jun;4(3):224-8 - PubMed
  46. Nat Biotechnol. 2005 Mar;23(3):297-304 - PubMed
  47. Life Sci. 1987 Aug 17;41(7):905-8 - PubMed
  48. J Nutr. 2003 Jun;133(6):1776-80 - PubMed
  49. Cancer Epidemiol Biomarkers Prev. 1996 Oct;5(10):811-4 - PubMed
  50. J Nutr. 2003 Nov;133(11 Suppl 1):3820S-3826S - PubMed
  51. J Nutr. 2003 Mar;133 Suppl 3:881S-887S - PubMed
  52. N Engl J Med. 2003 Apr 24;348(17):1625-38 - PubMed
  53. Ann Clin Biochem. 2007 Jul;44(Pt 4):343-52 - PubMed
  54. J Nutr. 2003 Mar;133 Suppl 3:875S-880S - PubMed
  55. Clin Chem. 1984 Sep;30(9):1477-88 - PubMed
  56. J Am Diet Assoc. 2007 May;107(5):760-7 - PubMed
  57. J Nutr Biochem. 2005 Apr;16(4):236-44 - PubMed
  58. Proc Natl Acad Sci U S A. 2004 May 18;101(20):7809-14 - PubMed
  59. Int J Epidemiol. 2004 Jun;33(3):518-25 - PubMed

Publication Types

Grant support