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Murooka Y, Yim MH, Harada T. Formation and Purification of Serratia marcescens Arylsulfatase. Appl Environ Microbiol. 1980;39(4):812-7doi: 10.1128/aem.39.4.812-817.1980.
Murooka, Y., Yim, M. H., & Harada, T. (1980). Formation and Purification of Serratia marcescens Arylsulfatase. Applied and environmental microbiology, 39(4), 812-7. https://doi.org/10.1128/aem.39.4.812-817.1980
Murooka, Y, et al. "Formation and Purification of Serratia marcescens Arylsulfatase." Applied and environmental microbiology vol. 39,4 (1980): 812-7. doi: https://doi.org/10.1128/aem.39.4.812-817.1980
Murooka Y, Yim MH, Harada T. Formation and Purification of Serratia marcescens Arylsulfatase. Appl Environ Microbiol. 1980 Apr;39(4):812-7. doi: 10.1128/aem.39.4.812-817.1980. PMID: 16345546; PMCID: PMC291424.
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Appl Environ Microbiol. 1980 Apr;39(4):812-7. doi: 10.1128/aem.39.4.812-817.1980.

Formation and Purification of Serratia marcescens Arylsulfatase.

Applied and environmental microbiology

Y Murooka, M H Yim, T Harada

Affiliations

  1. Institute of Scientific and Industrial Research, Osaka University, Suita-shi, Osaka 565, Japan.

PMID: 16345546 PMCID: PMC291424 DOI: 10.1128/aem.39.4.812-817.1980

Abstract

The effects of culture conditions on arylsulfatase production by six strains of the genus Serratia were studied. Synthesis of arylsulfatases in all six strains was repressed in media with inorganic sulfate or methionine as the sole source of sulfur and derepressed by the addition of tyramine. Serratia marcescens IFO 3046 grew most rapidly and produced a high level of arylsulfatase when cultured on mannitol with inorganic sulfate and tyramine. The derepressed synthesis of arylsulfatase in S. marcescens was not subject to strong catabolite repression. The molecular weight of purified arylsulfatase was determined to be between 46,000 and 49,000. Arylsulfatase from S. marcescens differed in K(m) and V(max) values, substrate specificities, fluoride inhibition, and electrophoretic mobility from the enzyme from K. aerogenes, but had the same molecular weight as the latter.

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